LOCUS       pRetroQ-AcGFP1-C1   7614 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632506
FEATURES             Location/Qualifiers
     misc_feature    1..727
                     /label=CMV/MSV\5'\LTR
     misc_feature    757..1566
                     /label=Extended\packaging\signal
     misc_feature    4313..4745
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(6392..7252)
                     /label=Amp(R)
     promoter        3000..3508
                     /label=PGK\Promoter
     CDS             3529..4128
                     /label=Puro(R)
     rep_origin      5246..5311
                     /label=SV40\origin
     promoter        1582..2170
                     /label=CMV\IE\Promoter
     CDS             2194..2910
                     /label=AcGFP1
     misc_feature    2187..2197
                     /label=Kozak\Sequence
     promoter        5025..5292
                     /label=SV40\Promoter
     rep_origin      5632..5632
                     /label=pUC\origin
     misc_feature    2920..2976
                     /label=MCS
BASE COUNT     1719 a      2183 c      1981 g      1731 t 
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca 
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc 
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc 
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg 
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg 
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg 
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc 
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac 
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc 
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc 
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc 
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc 
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatctcgagc tcaagcttcg 
     2941 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata 
     3001 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 
     3061 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 
     3121 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 
     3181 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 
     3241 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 
     3301 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 
     3361 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 
     3421 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 
     3481 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 
     3541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 
     3601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 
     3661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 
     3721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 
     3781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 
     3841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 
     3901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 
     3961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 
     4021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 
     4081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac 
     4141 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggcgtc tcgatcgaga 
     4201 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt 
     4261 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag 
     4321 accccacctg taggtttggc aagctactag cttaagtaac gccattttgc aaggcatgga 
     4381 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt 
     4441 cgatcgaccc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc 
     4501 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct 
     4561 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac 
     4621 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg 
     4681 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt 
     4741 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc 
     4801 gggaggtaag ctggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 
     4861 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 
     4921 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 
     4981 gatagcggag tgtagatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 
     5041 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 
     5101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     5161 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 
     5221 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 
     5281 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 
     5341 aagcttactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 
     5401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 
     5461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     5521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     5581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     5641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     5701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     5761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     5821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     5881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     5941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     6001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     6061 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     6121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     6181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     6241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     6301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     6361 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     6421 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     6481 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     6541 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     6601 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     6661 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg 
     6721 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     6781 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     6841 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     6901 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     6961 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata 
     7021 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     7081 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     7141 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     7201 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     7261 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     7321 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     7381 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 
     7441 ggccctttcg tcttcaagaa ttagcttggc cattgcatac gttgtatcca tatcataata 
     7501 tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga ttattgacta 
     7561 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagt 
//
