LOCUS       pQCLIN Retroviral Vector               10764 bp ds-DNA     circular SYN 17-APR-2020
COMMENT    Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT    631516, 631457

FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..728
                     /label=5' CMV/MSV LTR
     misc_feature    758..1567
                     /label=MMLV Psi+
     promoter        1601..2132
                     /label=CMV promoter
     CDS             2434..5481
                     /label=lacZ
     misc_feature    5672..6245
                     /label=IRES
     CDS             6259..7053
                     /label=NeoR/KanR
     LTR             7470..7895
                     /label=3' LTR (Delta-U3)
     rep_origin      complement(8783..9371)
                     /direction=LEFT
                     /label=ori
     CDS             complement(9542..10402)
                     /label=AmpR
     promoter        complement(10403..10507)
                     /label=AmpR promoter
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgct ctagaactag tatctataag aggaagaggg
     2281 tgctggctcc caggccacag cccacaaaat tccacctgct cacaggttgg ctggctcgac
     2341 ccaggtggtg tcccctgctc tgagccagct cccggccaag ccagcaccat ggggcccaag
     2401 aagaaacgca aagtggggag catgggggat cccgtcgttt tacaacgtcg tgactgggaa
     2461 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2521 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2581 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
     2641 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
     2701 cccatctaca ccaacgtgac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
     2761 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
     2821 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
     2881 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
     2941 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct ggagtgacgg cagttatctg
     3001 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa
     3061 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
     3121 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
     3181 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
     3241 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     3301 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     3361 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     3421 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     3481 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     3541 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     3601 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     3661 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     3721 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     3781 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     3841 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     3901 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     3961 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     4021 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     4081 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     4141 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     4201 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     4261 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     4321 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg
     4381 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     4441 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     4501 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     4561 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     4621 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     4681 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     4741 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     4801 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     4861 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     4921 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     4981 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     5041 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     5101 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     5161 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     5221 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     5281 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     5341 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     5401 tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta ccattaccag
     5461 ttggtctggt gtcaaaaata ataataaccg ggcagggggg atccgcagat cccggccaga
     5521 taccgatgct gccgcagcaa aagcaggagc agatgccgcc gtcgcaggcg aagatgtcgc
     5581 agacggagga ggcgatgctg ccatcaagct tatcgatacc gtcgacctcg agggggggcc
     5641 cggtacgaat tccgcccccc cccccccccc ccccctctcc ctcccccccc cctaacgtta
     5701 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5761 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5821 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5881 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5941 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     6001 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     6061 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     6121 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     6181 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6241 gataagcttg ccacaaccat ggctgaacaa gatggattgc acgcaggttc tccggccgct
     6301 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6361 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6421 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6481 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6541 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6601 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6661 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6721 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6781 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     6841 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     6901 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6961 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7021 gccttctatc gccttcttga cgagttcttc tgagcgggat cggctagagt cgacccggac
     7081 gggacgctcc tgcgcctgat acagaacgaa ttgcttgcag gcatctcatg agtgtgtctt
     7141 cccgttttcc gcctgaggtc actgcgtgga tggagcgctg gcgcctgctg cgcgacggcg
     7201 agctgctcac cacccactcg agggctgcag cgctgcagag gccgagtgca gaactgctcc
     7261 aaagggacct caaggctttc cgagggacac taggctgact ccatcgagcc agtgtagaga
     7321 taagcttatc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
     7381 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag
     7441 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     7501 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     7561 ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt
     7621 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     7681 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     7741 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     7801 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     7861 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     7921 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc
     7981 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     8041 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     8101 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat
     8161 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8221 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8281 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8461 ggcttttttg gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag
     8521 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8581 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8641 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8701 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8761 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8821 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8881 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8941 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9001 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9061 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9121 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9181 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9241 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9301 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9361 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9421 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9481 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9541 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9601 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9661 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9721 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9781 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9841 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9901 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9961 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10021 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10081 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10141 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10201 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10261 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10321 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10381 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10441 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10501 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10561 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc
    10621 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
    10681 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
    10741 tagttcatag cccatatatg gagt
//
