LOCUS pPAmCherry-Mem 4776 bp DNA circular SYN 17-JAN-2012 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No.632588 FEATURES Location/Qualifiers source 1..4776 /organism="recombinant plasmid" /mol_type="other DNA" promoter 1..589 /label=pCMV /note="color: #66cc99" CDS 732..1439 /label=PAmCherry /note="color: #ff9966" rep_origin complement(1692..2147) /label=f1\ori /note="color: #ff9966" rep_origin 2488..2626 /label=SV40\ori /note="color: #ff9966" CDS 2672..3466 /label=Kan(R)/Neo(R) /note="color: #ff9966" rep_origin 4051..4694 /label=pUC\ori /note="color: #ff9966" CDS 672..731 /label=Membrane-targeting\sequence polyA_signal 3702..3720 /label=HSV\TK\polyA\signal polyA_signal 1595..1629 /label=SV40\poly\A promoter 2321..2589 /label=pSV40_e BASE COUNT 1155 a 1270 c 1276 g 1075 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccgccac catgctgtgc tgtatgagaa gaaccaaaca ggttgaaaag aatgatgagg 721 accaaaagat catggtgagc aagggcgagg aggataacat ggccatcatt aaggagttca 781 tgcgcttcaa ggtgcacatg gaggggtccg tgaacggcca cgtgttcgag atcgagggcg 841 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 901 gccccctgcc cttcacctgg gacatcctgt cccctcaatt catgtacggc tccaatgcct 961 acgtgaagca ccccgccgac atccccgact actttaagct gtccttcccc gagggcttca 1021 agtgggagcg cgtgatgaaa ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 1081 ccctgcagga cggtgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1141 acggccccgt aatgcagaag aagaccatgg gctgggaggc cctctccgag cggatgtacc 1201 ccgaggacgg cgccctgaag ggcgaggtca agccgagagt gaagctgaag gacggcggcc 1261 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1321 cctacaacgt caaccgcaag ttggacatca cctcacacaa cgaggactac accatcgtgg 1381 aacagtacga acgtgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 1441 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct 1501 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 1561 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 1621 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 1681 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 1741 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 1801 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 1861 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 1921 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 1981 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 2041 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 2101 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt 2161 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 2221 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc 2281 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2341 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2401 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2461 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2521 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 2581 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat 2641 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 2701 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 2761 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 2821 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 2881 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 2941 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 3001 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 3061 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 3121 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 3181 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 3241 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 3301 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 3361 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 3421 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 3481 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 3541 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 3601 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca 3661 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3721 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct 3781 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca 3841 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc 3901 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa 3961 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4021 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 4141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 4201 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 4261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 4321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 4381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 4441 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 4501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 4561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 4621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 4681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 4741 cctgattctg tggataaccg tattaccgcc atgcat //