LOCUS pPAmCherry-Actin 5810 bp DNA circular SYN 2-AUG-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No.632589 FEATURES Location/Qualifiers source 1..5810 /organism="recombinant plasmid" /mol_type="other DNA" promoter 1..589 /label=pCMV\IE /note="color: #66cc99" CDS 612..1319 /label=PAmCherry /note="color: #ff9966" CDS 1341..2465 /label=Actin /note="color: #ff9966" rep_origin complement(2726..3181) /label=f1\ori /note="color: #ff9966" rep_origin 3522..3660 /label=SV40\ori /note="color: #ff9966" CDS 3706..4500 /label=Kan(R)/Neo(R) /note="color: #ff9966" rep_origin 5085..5728 /label=pUC\ori /note="color: #ff9966" polyA_signal 4736..4754 /label=HSV\TK\polyA\signals promoter 3355..3623 /label=pSV40_e polyA_signal 2629..2663 /label=SV40\poly\A BASE COUNT 1360 a 1623 c 1557 g 1270 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtgccac catggtgagc aagggcgagg aggataacat ggccatcatt aaggagttca 661 tgcgcttcaa ggtgcacatg gaggggtccg tgaacggcca cgtgttcgag atcgagggcg 721 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 781 gccccctgcc cttcacctgg gacatcctgt cccctcaatt catgtacggc tccaatgcct 841 acgtgaagca ccccgccgac atccccgact actttaagct gtccttcccc gagggcttca 901 agtgggagcg cgtgatgaaa ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 961 ccctgcagga cggtgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1021 acggccccgt aatgcagaag aagaccatgg gctgggaggc cctctccgag cggatgtacc 1081 ccgaggacgg cgccctgaag ggcgaggtca agccgagagt gaagctgaag gacggcggcc 1141 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1201 cctacaacgt caaccgcaag ttggacatca cctcacacaa cgaggactac accatcgtgg 1261 aacagtacga acgtgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 1321 ccggactcag atctcgagct atggatgatg atatcgccgc gctcgtcgtc gacaacggct 1381 ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca 1441 tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag gattcctatg 1501 tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc atcgagcacg 1561 gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc tacaatgagc 1621 tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg aaccccaagg 1681 ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca gccatgtacg 1741 ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc atcgtgatgg 1801 actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc ctcccccatg 1861 ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg aagatcctca 1921 ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt gacattaagg 1981 agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct gcttccagct 2041 cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt ggcaatgagc 2101 ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag tcctgtggca 2161 tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc aaagacctgt 2221 acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc gacaggatgc 2281 agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt gctcctcctg 2341 agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc accttccagc 2401 agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc caccgcaaat 2461 gcttctaggg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag 2521 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 2581 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2701 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 2761 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 2821 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 2881 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 2941 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 3001 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 3061 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 3121 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 3181 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 3241 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 3301 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 3361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 3541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 3601 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 3661 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 3721 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 3781 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 3841 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 3901 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 3961 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4021 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4081 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4141 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4201 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 4261 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4321 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4381 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4441 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4501 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 4561 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 4621 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 4681 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 4741 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 4801 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 4861 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 4921 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 4981 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5041 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 5101 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 5161 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 5221 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 5281 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 5341 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 5401 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 5461 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 5521 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 5581 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 5641 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 5701 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 5761 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat //