LOCUS       pNY326 Vector 		3438 bp DNA     circular SYN 20-AUG-2021
COMMENT     Created by Takara Bio Inc. http://www.takarabio.com
COMMENT     Cat. No. HB111
FEATURES             Location/Qualifiers
     promoter        1..161            
                     /label="P5 promoter"
     misc_feature    250..313
                     /label="MCS"
                     /note="multiple cloning site"
     misc_feature    162..251
                     /label="Secretion signal peptide"
     CDS             875..1879
                     /label="RepB"
                     /note="RepB replication protein from Enterococcus faecalis plasmid pAM-alpha-1"
     CDS             2048..2809                     
                     /label="NmR"
                     /note="Neomycin resistance gene, selection marker in Brevibacillus"
ORIGIN
        1 caggggaata tactagagat ttttaacaca aaaagcgagg ctttcctgcg aaaggaggtg
       61 acacgcgctt gcaggattcg ggctttaaaa agaaagatag attaacaaca aatattcccc
      121 aagaacaatt tgtttatact agaggaggag aacacaaggt catgaaaaaa agaagggtcg
      181 ttaacagtgt attgcttctg ctactgctag ctagtgcact cgcacttact gttgctccca
      241 tggctttcgc tgcaggatcc gtcgactcta gactcgagga attcggtacc ccgggttcga
      301 aatcgataag cttaacagga tgcgggggag ccgccgctcc gtcgccccct gcggggggct
      361 tccgtatgcg gcaggcatcc cctcgcatgc aggtagggaa caattacatt gtctttgatt
      421 gtaaaaatgc tgttgacagg acactaaatg gtgtcgtatt ctcaaagtaa caccatttgg
      481 tgtccaattg caagtcattt ggtaacctta attggatcag acaaggtaaa ggataaaaca
      541 gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga acgaatttga actaactcat
      601 aaccgagagg taaaaaaaga acgaagtcga gatcagggaa tgagtttata aaataaaaaa
      661 agcacctgaa aaggtgtctt tttttgatgg ttttgaactt gttctttctt atcttgatac
      721 atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag tgttggtatg
      781 tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac cccatctgtt
      841 aaagttataa gtgactaaac aaataactaa atagatgggg gtttctttta atattatgtg
      901 tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca agacaaaaag
      961 tggaaaagtg aggccttgga gagaaaagaa aatcgctaat gttgattact ttgaacttct
     1021 gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa tattagagta
     1081 taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag
     1141 gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt cacaaaaggt
     1201 tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca cattaacagt
     1261 taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg ctcaaggatt
     1321 tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac
     1381 ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg tattggtatg
     1441 tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac aatggattca
     1501 attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc aaatgattcg
     1561 accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg caaaatatcc
     1621 tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac gtttgtctga
     1681 tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat
     1741 acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata cagatgatga
     1801 cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg aacggaaaaa
     1861 ttattttatt aaagagtagt tcaacaaacg ggccagtttg ttgaagatta gatgctataa
     1921 ttgttattaa aaggattgaa ggatgcttag gaagacgagt tattaatagc tgaataagaa
     1981 cggtgctctc caaatattct tatttagaaa agcaaatcta aaattatctg aaaagggaat
     2041 gagaatagtg aatggaccaa taataatgac tagagaagaa agaatgaaga ttgtccatga
     2101 aattaaggaa cgaatattgg ataaatatgg ggatgatgtt aaggctattg gtgtttatgg
     2161 ctctcttggt cgtcagactg atgggcccta ttcggatatt gagatgatgt gtgtcatgtc
     2221 aacagaggaa gcagagttca gccatgaatg gacaaccggt gagtggaagg tggaagtgaa
     2281 ttttgatagc gaagagattc tactagatta tgcatctcag gtggaatcag attggccgct
     2341 tacacatggt caatttttct ctattttgcc gatttatgat tcaggtggat acttagagaa
     2401 agtgtatcaa actgctaaat cggtagaagc ccaaacgttc cacgatgcga tttgtgccct
     2461 tatcgtagaa gagctgtttg aatatgcagg caaatggcgt aatattcgtg tgcaaggacc
     2521 gacaacattt ctaccatcct tgactgtaca ggtagcaatg gcaggtgcca tgttgattgg
     2581 tctgcatcat cgcatctgtt atacgacgag cgcttcggtc ttaactgaag cagttaagca
     2641 atcagatctt ccttcaggtt atgaccatct gtgccagttc gtaatgtctg gtcaactttc
     2701 cgactctgag aaacttctgg aatcgctaga gaatttctgg aatgggattc aggagtggac
     2761 agaacgacac ggatatatag tggatgtgtc aaaacgcata ccattttgaa cgatgacctc
     2821 taataattgt taatcatgtt ggttacgtat ttattaactt ctcctagtat tagtaattat
     2881 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cggctgaata
     2941 aaagatacga gagacctctc ttgtatcttt tttattttga gtggttttgt ccgttacact
     3001 agaaaaccga aagacaataa aaattttatt cttgctgagt ctggctttcg gtaagctaga
     3061 caaaacggac aaaataaaaa ttggcaaggg tttaaaggtg gagatttttt gagtgatctt
     3121 ctcaaaaaat actacctgtc ccttgctgat ttttaaacga gcacgagagc aaaacccccc
     3181 tttgctgagg tggcagaggg caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag
     3241 gtcttaaagg ttttatggtt ttggtcggca ctgccgacag cctcgcagag cacacacttt
     3301 atgaatataa agtatagtgt gttatacttt acttggaagt ggttgccgga aagagcgaaa
     3361 atgcctcaca tttgtgccac ctaaaaagga gcgatttaca tatgagttat gcagtttgta
     3421 gaatgcaaaa agtgaaat
//
