LOCUS       pNI Vector		5055 bp DNA     circular SYN 20-AUG-2021
COMMENT     Created by Takara Bio Inc. http://www.takarabio.com
COMMENT     Cat. No. HB131
FEATURES             Location/Qualifiers
     protein_bind    104..125
                     /label="Lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             756..1760
                     /label="RepB"
                     /note="RepB replication proteinfrom Enterococcus faecalis plasmid pAM-alpha-1"
     promoter        2913..3017
                     /label=AmpR promoter
     promoter        61..103            
                     /label=P2 promoter
     misc_feature    164..240
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             3018..3878
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      4049..4637
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1929..2690                     
                     /label="NmR"
                     /note="Neomycin resistance gene, selection marker in Brevibacillus"
ORIGIN
        1 tgatccgaca taatggacag gtgaataacg aaccacgaaa aaaactttaa atttttttcg
       61 aaggcgccgc aacttttgat tcgctcaggc gtttaatagg atgtaattgt gagcggataa
      121 caattattct gcatggcttt cctgcgaaag gaggtgacac gcgccatggc tttcgctgca
      181 ggatccgtcg actctagact cgaggaattc ggtaccccgg gttcgaaatc gataagcttc
      241 ggcattatag tgcggaggct ttttcgcatg caggtaggga acaattacat tgtctttgat
      301 tgtaaaaatg ctgttgacag gacactaaat ggtgtcgtat tctcaaagta acaccatttg
      361 gtgtccaatt gcaagtcatt tggtaacctt aattggatca gacaaggtaa aggataaaac
      421 agcacaattc caagaaaaac acgatttaga acctaaaaag aacgaatttg aactaactca
      481 taaccgagag gtaaaaaaag aacgaagtcg agatcaggga atgagtttat aaaataaaaa
      541 aagcacctga aaaggtgtct ttttttgatg gttttgaact tgttctttct tatcttgata
      601 catatagaaa taacgtcatt tttattttag ttgctgaaag gtgcgttgaa gtgttggtat
      661 gtatgtgttt taaagtattg aaaaccctta aaattggttg cacagaaaaa ccccatctgt
      721 taaagttata agtgactaaa caaataacta aatagatggg ggtttctttt aatattatgt
      781 gtcctaatag tagcatttat tcagatgaaa aatcaagggt tttagtggac aagacaaaaa
      841 gtggaaaagt gagaccttgg agagaaaaga aaatcgctaa tgttgattac tttgaacttc
      901 tgcatattct tgaatttaaa aaggctgaaa gagtaaaaga ttgtgctgaa atattagagt
      961 ataaacaaaa tcgtgaaaca ggcgaaagaa agttgtatcg agtgtggttt tgtaaatcca
     1021 ggctttgtcc aatgtgcaac tggaggagag caatgaaaca tggcattcag tcacaaaagg
     1081 ttgttgctga agttattaaa caaaagccaa cagttcgttg gttgtttctc acattaacag
     1141 ttaaaaatgt ttatgatggc gaagaattaa ataagagttt gtcagatatg gctcaaggat
     1201 ttcgccgaat gatgcaatat aaaaaaatta ataaaaatct tgttggtttt atgcgtgcaa
     1261 cggaagtgac aataaataat aaagataatt cttataatca gcacatgcat gtattggtat
     1321 gtgtggaacc aacttatttt aagaatacag aaaactacgt gaatcaaaaa caatggattc
     1381 aattttggaa aaaggcaatg aaattagact atgatccaaa tgtaaaagtt caaatgattc
     1441 gaccgaaaaa taaatataaa tcggatatac aatcggcaat tgacgaaact gcaaaatatc
     1501 ctgtaaagga tacggatttt atgaccgatg atgaagaaaa gaatttgaaa cgtttgtctg
     1561 atttggagga aggtttacac cgtaaaaggt taatctccta tggtggtttg ttaaaagaaa
     1621 tacataaaaa attaaacctt gatgacacag aagaaggcga tttgattcat acagatgatg
     1681 acgaaaaagc cgatgaagat ggattttcta ttattgcaat gtggaattgg gaacggaaaa
     1741 attattttat taaagagtag ttcaacaaac gggccagttt gttgaagatt agatgctata
     1801 attgttatta aaaggattga aggatgctta ggaagacgag ttattaatag ctgaataaga
     1861 acggtgctct ccaaatattc ttatttagaa aagcaaatct aaaattatct gaaaagggaa
     1921 tgagaatagt gaatggacca ataataatga ctagagaaga aagaatgaag attgtccatg
     1981 aaattaagga acgaatattg gataaatatg gggatgatgt taaggctatt ggtgtttatg
     2041 gctctcttgg tcgtcagact gatgggccct attcggatat tgagatgatg tgtgtcatgt
     2101 caacagagga agcagagttc agccatgaat ggacaaccgg tgagtggaag gtggaagtga
     2161 attttgatag cgaagagatt ctactagatt atgcatctca ggtggaatca gattggccgc
     2221 ttacacatgg tcaatttttc tctattttgc cgatttatga ttcaggtgga tacttagaga
     2281 aagtgtatca aactgctaaa tcggtagaag cccaaacgtt ccacgatgcg atttgtgccc
     2341 ttatcgtaga agagctgttt gaatatgcag gcaaatggcg taatattcgt gtgcaaggac
     2401 cgacaacatt tctaccatcc ttgactgtac aggtagcaat ggcaggtgcc atgttgattg
     2461 gtctgcatca tcgcatctgt tatacgacga gcgcttcggt cttaactgaa gcagttaagc
     2521 aatcagatct tccttcaggt tatgaccatc tgtgccagtt cgtaatgtct ggtcaacttt
     2581 ccgactctga gaaacttctg gaatcgctag agaatttctg gaatgggatt caggagtgga
     2641 cagaacgaca cggatatata gtggatgtgt caaaacgcat accattttga acgatgacct
     2701 ctaataattg ttaatcatgt tggttacgta tttattaact tctcctagta ttagtaatta
     2761 tcatggctgt catggcgcat taacggaata aagggtgtgc ttaaatcggg cctgcatgac
     2821 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     2881 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2941 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3001 attgaaaaag gaagagtatg agtattcaac atttccgtgt cccccttatt cccttttttg
     3061 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3121 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3181 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3241 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3301 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     3361 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     3421 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     3481 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     3541 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3601 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3661 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3721 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3781 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3841 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3901 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3961 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4021 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4081 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     4201 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4261 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4321 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4381 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4441 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4501 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4561 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4621 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4681 ggccatgcag gctgaataaa agatacgaga gacctctctt gtatcttttt tattttgagt
     4741 ggttttgtcc gttacactag aaaaccgaaa gacaataaaa attttattct tgctgagtct
     4801 ggctttcggt aagctagaca aaacggacaa aataaaaatt ggcaagggtt taaaggtgga
     4861 gattttttga gtgatcttct caaaaaatac tacctgtccc ttgctgattt ttaaacgagc
     4921 acgagagcaa aacccccctt tgctgaggtg gcagagggca ggtttttttg tttctttttt
     4981 ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt ggtcggcact gccgacagcc
     5041 tcgcagagca cacac
//
