LOCUS       pMEI-5 Vector 	4689 bp DNA     circular SYN 19-AUG-2021
COMMENT     Created by Takara Bio Inc. http://www.takarabio.com
COMMENT     Cat No. 3656
FEATURES             Location/Qualifiers
     5'-LTR          528..997
                     /label="5'LTR"
     misc_feature    1062..1419
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_feature    1760..1816
                     /label="MSC"
                     /note="multiple cloning site"
     intron          1482..1685
                     /label="EF1alpha intron A"
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     exon            1686..1715
                     /label="EF1alpha exon"
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     3'-LTR          1859..2452
                     /label="3'LTR"
     rep_origin      complement(2870..3458)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3629..4489)
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtga aagaccccac
      421 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
      481 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      541 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      601 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      661 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      721 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      781 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccgg gctcaataaa agagcccaca
      841 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      901 aataaaccct cttgcagttg catccgactt gtggcctcgc tgttccttgg gagggtctcc
      961 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
     1021 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
     1081 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
     1141 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
     1201 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
     1261 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt
     1321 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
     1381 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1441 ctgtctgact gtgtttctgt atttgtctga aaatacgcgt gtcgagcttt tggagtacgt
     1501 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1561 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1621 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1681 ttcagccgtc gtgaaaacta cccctaaaag ccaaaaaaat cgaattccca aacttaagct
     1741 tggtaccgag ctcggatctc acgtgggccc gcggccgcag atctatcgat ggatccgtcg
     1801 acgttaactg ctcgaggata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     1861 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     1921 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     1981 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     2041 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     2101 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     2161 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     2221 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     2281 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     2341 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     2401 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca ttgaattcgt
     2461 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2521 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2581 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2641 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     2701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     4321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     4381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     4441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     4501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     4561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     4681 cctttcgtc
//
