LOCUS       pLacZ-Basic   7483 bp    DNA   circular            11-MAR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631707,631713
FEATURES             Location/Qualifiers
     misc_feature    1..52
                     /label=MCS
     misc_feature    94..3237
                     /label=B-galactosidase\fusion\protein
     misc_feature    3854..3919
                     /label=SV40\intron
     misc_feature    4525..4559
                     /label=SV40\early\polyA\signals
     misc_feature    4909..5552
                     /label=pUC\origin
     misc_feature    complement(5700..6560)
                     /label=Amp(R)
     misc_feature    6692..7147
                     /label=f1\origin
     misc_feature    7368..7402
                     /label=SV40\early\polyA\signals
     misc_feature    94..186
                     /label=aa.s\from\D.\melanogaster\alcohol\dehydrogenase
     misc_feature    190..3234
                     /label=aa.s\from\E.\coli\B-galactosidase\(LacZ)
BASE COUNT     1859 a      1864 c      1890 g      1870 t 
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttgggggatc 
       61 gaaagagcct gctaaagcaa aaaagaagtc accatgtcgt ttactttgac caacaagaac 
      121 gtgattttcg ttgccggtct gggaggcatt ggtctggaca ccagcaagga gctgctcaag 
      181 cgcgatcccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 
      241 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 
      301 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca 
      361 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc 
      421 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat 
      481 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc 
      541 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc 
      601 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt 
      661 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg 
      721 gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg 
      781 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc 
      841 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag 
      901 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg 
      961 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat 
     1021 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc 
     1081 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca 
     1141 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac 
     1201 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag 
     1261 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac 
     1321 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac 
     1381 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt 
     1441 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag 
     1501 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc 
     1561 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag 
     1621 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc 
     1681 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg 
     1741 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt 
     1801 cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt atccccgttt acagggcggc 
     1861 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg 
     1921 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt 
     1981 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag 
     2041 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt 
     2101 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc 
     2161 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta 
     2221 ccgcagccgg agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg 
     2281 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac 
     2341 ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc atctgaccac cagcgaaatg 
     2401 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt 
     2461 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc 
     2521 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc 
     2581 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc 
     2641 acggcagata cacttgctga tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag 
     2701 gggaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg 
     2761 attaccgttg atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgaac 
     2821 tgccagctgg cgcaggtagc agagcgggta aactggctcg gattagggcc gcaagaaaac 
     2881 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg 
     2941 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat 
     3001 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagtcaacag 
     3061 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat 
     3121 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg 
     3181 gaattacagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa 
     3241 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta 
     3301 tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc 
     3361 gggcgtggca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt 
     3421 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac 
     3481 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac 
     3541 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa 
     3601 gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatac 
     3661 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg 
     3721 cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgccggat 
     3781 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat 
     3841 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct 
     3901 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa 
     3961 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc 
     4021 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa 
     4081 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct 
     4141 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat 
     4201 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt 
     4261 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg 
     4321 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt 
     4381 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 
     4441 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 
     4501 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     4561 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     4621 tctggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 
     4681 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag 
     4741 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     4801 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     4861 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     4921 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     4981 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     5041 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     5101 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     5161 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     5221 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     5281 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     5341 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     5401 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     5461 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     5521 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     5581 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     5641 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     5701 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     5761 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     5821 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     5881 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     5941 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     6001 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     6061 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     6121 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     6181 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     6241 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     6301 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     6361 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     6421 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     6481 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     6541 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     6601 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     6661 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg 
     6721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 
     6781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 
     6841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 
     6901 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 
     6961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 
     7021 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 
     7081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 
     7141 acaatttgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 
     7201 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgtgga 
     7261 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     7321 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     7381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 
     7441 actcatcaat gtatcttatg gtactgtaac tgagctaaca taa 
//
