LOCUS       pLVX-Tight-Puro   7790 bp    DNA   circular             9-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632158,632162,632157,632163
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     misc_feature    3712..4303
                     /label=WPRE
     rep_origin      complement(5611..6284)
                     /label=pUC\origin
     CDS             complement(6429..7425)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             4506..5142
                     /label=3'\LTR
     promoter        2205..2520
                     /label=TREtight\Promoter
     promoter        2572..3075
                     /label=PGK\Promoter
     CDS             3096..3695
                     /label=Puro(R)
BASE COUNT     1959 a      1941 c      2046 g      1844 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt 
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa 
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca 
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt 
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     2521 tggagaagga tccgcggccg cgccggctct agatcgcgaa cgcgtgaatt ctaccgggta 
     2581 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact 
     2641 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa 
     2701 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag 
     2761 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc 
     2821 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc 
     2881 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg 
     2941 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga 
     3001 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca 
     3061 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc 
     3121 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 
     3181 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 
     3241 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 
     3301 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 
     3361 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 
     3421 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 
     3481 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 
     3541 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 
     3601 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 
     3661 gcacctggtg catgacccgc aagcccggtg cctgacgggc gcgtctggaa caatcaacct 
     3721 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     3781 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     3841 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     3901 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     3961 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     4021 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     4081 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     4141 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     4201 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     4261 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc 
     4321 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt 
     4381 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac 
     4441 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga 
     4501 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct 
     4561 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat 
     4621 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag 
     4681 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg 
     4741 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg 
     4801 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac 
     4861 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc 
     4921 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag 
     4981 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 
     5041 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 
     5101 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt 
     5161 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat 
     5221 tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 
     5281 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     5341 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     5401 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     5461 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     5521 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     5581 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     5641 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     5701 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     5761 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     5821 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     5881 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     5941 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     6001 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     6061 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     6121 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     6181 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     6241 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     6301 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     6361 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     6421 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     6481 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     6541 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     6601 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     6661 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     6721 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     6781 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     6841 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     6901 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     6961 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     7021 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     7081 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     7141 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     7201 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     7261 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     7321 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     7381 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 
     7441 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     7501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     7561 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 
     7621 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc 
     7681 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt 
     7741 agtttttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt 
//
