LOCUS       pLVX-Tight-Puro-Luc   9423 bp    DNA   circular             9-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632162,632158,632163,632157
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     misc_feature    5345..5936
                     /label=WPRE
     rep_origin      complement(7244..7917)
                     /label=pUC\origin
     CDS             complement(8062..9058)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             6139..6775
                     /label=3'\LTR
     promoter        2205..2520
                     /label=TREtight\Promoter
     promoter        4205..4708
                     /label=PGK\promoter
     CDS             4729..5328
                     /label=Puro(R)
     misc_feature    2537..4189
                     /label=Luciferase
BASE COUNT     2437 a      2281 c      2432 g      2273 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt 
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa 
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca 
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt 
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     2521 tggagaagga tccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta 
     2581 tccgctagag gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct 
     2641 ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca cgtacgcgga 
     2701 atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa 
     2761 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc 
     2821 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct 
     2881 caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa 
     2941 aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca tggattctaa 
     3001 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg 
     3061 ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa ttgcactgat 
     3121 aatgaactcc tctggatcta ctgggttacc taagggtgtg gcccttccgc atagaactgc 
     3181 ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac 
     3241 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata 
     3301 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttttacg 
     3361 atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat tttcattctt 
     3421 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg 
     3481 gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc atcttccagg 
     3541 gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg 
     3601 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga 
     3661 tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg tcagaggacc 
     3721 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga 
     3781 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatagt 
     3841 tgaccgcttg aagtctttaa ttaaatacaa aggataccag gtggcccccg ctgaattgga 
     3901 gtcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc ttcccgacga 
     3961 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga 
     4021 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg 
     4081 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat 
     4141 cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaaa atacgcgtga 
     4201 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 
     4261 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 
     4321 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 
     4381 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 
     4441 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 
     4501 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 
     4561 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 
     4621 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 
     4681 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 
     4741 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 
     4801 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 
     4861 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 
     4921 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 
     4981 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 
     5041 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 
     5101 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 
     5161 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 
     5221 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 
     5281 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg ggcgcgtctg 
     5341 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 
     5401 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 
     5461 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 
     5521 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 
     5581 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 
     5641 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 
     5701 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct 
     5761 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 
     5821 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 
     5881 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat 
     5941 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct 
     6001 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc 
     6061 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 
     6121 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt 
     6181 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg 
     6241 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca 
     6301 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg 
     6361 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca 
     6421 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct 
     6481 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg 
     6541 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct 
     6601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
     6661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
     6721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 
     6781 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 
     6841 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg 
     6901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     6961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     7021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     7081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     7141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     7201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     7261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     7321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     7381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     7441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     7501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     7561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     7621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     7681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     7741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     7801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     7861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     7921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     7981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     8041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     8101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     8161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     8221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     8281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     8341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     8401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     8461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     8521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     8581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     8641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     8701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     8761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     8821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     8881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     8941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     9001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga 
     9061 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 
     9121 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 
     9181 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc 
     9241 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct 
     9301 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta 
     9361 ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta caaacttagt 
     9421 agt 
//
