LOCUS pLVX-TetOne-Puro Vector 9180 bp ds-DNA circular DNA 08-MAY-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT Catalog Nos. 631847, 631848, 631849 SOURCE synthetic DNA construct FEATURES Location/Qualifiers LTR 1..634 /label=3' LTR misc_feature 681..806 /label=HIV-1 Psi packaging signal misc_feature 1303..1536 /label=RRE misc_feature 2028..2143 /label=cPPT/CTS polyA_signal 2187..2321 /label=SV40 poly(A) signal misc_feature complement(2496..2527) /label="MCS" promoter complement(2528..2895) /label=TRE3GS promoter promoter 2912..3422 /label=hPGK promoter CDS 3441..4187 /label=Tet-On 3G promoter 4198..4527 /label=SV40 promoter CDS 4536..5135 /label=PuroR misc_feature 5149..5737 /label=WPRE LTR 5944..6577 /label=3' LTR rep_origin complement(7107..7695) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7866..8726) /label=AmpR promoter complement(8727..8831) /label=AmpR promoter ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat 2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg 2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg 2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca 2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat 2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg 2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg 2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca 2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg 2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt 2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa 2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg 2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca 2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg 3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg 3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct 3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg 3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata 3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg 3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc 3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc 3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc 3481 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc 3541 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg 3601 ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt 3661 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc 3721 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg 3781 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc 3841 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag 3901 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa 3961 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca 4021 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg 4081 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc 4141 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg cgcgaatgtg 4201 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4261 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 4321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 4381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 4441 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 4501 ttttttggag gcctaggctt ttgcaaaacg cgaccatgac cgagtacaag cccacggtgc 4561 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg 4621 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc 4681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 4741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 4801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 4861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 4921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 4981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 5041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 5101 gcacctggtg catgacccgc aagcccggtg cctgaacgcg tctggaacaa tcaacctctg 5161 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5221 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5281 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5341 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5401 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5461 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5521 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5581 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5641 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 5701 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag 5761 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg 5821 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt 5881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg 5941 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc 6001 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 6061 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 6121 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 6181 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 6241 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 6301 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 6361 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc 6661 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 6721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 6781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 6841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 6901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 6961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 7561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 7681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 7741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 7801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 7861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 7921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 7981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 8221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 8281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 8341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 8401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 8461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 8521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 8581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 8641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 8701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 8761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 8821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa 8881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 8941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 9001 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc 9061 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc 9121 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt //