LOCUS       pLVX-TetOne-Puro Vector                9180 bp ds-DNA     circular DNA 08-MAY-2019
COMMENT     Created by Takara Bio USA, Inc.        http://www.takarabio.com
COMMENT     Catalog Nos. 631847, 631848, 631849
SOURCE      synthetic DNA construct

FEATURES             Location/Qualifiers
     LTR             1..634
                     /label=3' LTR
     misc_feature    681..806
                     /label=HIV-1 Psi packaging signal
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2143
                     /label=cPPT/CTS
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
     misc_feature    complement(2496..2527)
                     /label="MCS"
     promoter        complement(2528..2895)
                     /label=TRE3GS promoter
     promoter        2912..3422
                     /label=hPGK promoter
     CDS             3441..4187
                     /label=Tet-On 3G
     promoter        4198..4527
                     /label=SV40 promoter
     CDS             4536..5135
                     /label=PuroR
     misc_feature    5149..5737
                     /label=WPRE
     LTR             5944..6577
                     /label=3' LTR
     rep_origin      complement(7107..7695)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7866..8726)
                     /label=AmpR
     promoter        complement(8727..8831)
                     /label=AmpR promoter
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtgtatacg
     2521 ggaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa aagtgttgtg
     2581 gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt tacataagca
     2641 aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc tctatcactg
     2701 atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact ggtcatacgt
     2761 tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat agggagtaaa
     2821 gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc tctatcactg
     2881 atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt ttccaaggca
     2941 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     3001 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     3061 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     3121 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     3181 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     3241 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     3301 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     3361 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     3421 agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata aactctgctc
     3481 tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     3541 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     3601 ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg gaaggcgagt
     3661 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     3721 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     3781 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     3841 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     3901 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg aaacaagcaa
     3961 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     4021 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg
     4081 attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc
     4141 ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg cgcgaatgtg
     4201 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4321 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4441 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4501 ttttttggag gcctaggctt ttgcaaaacg cgaccatgac cgagtacaag cccacggtgc
     4561 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     4621 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     4681 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     4741 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     4801 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     4861 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     4921 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     4981 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     5041 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     5101 gcacctggtg catgacccgc aagcccggtg cctgaacgcg tctggaacaa tcaacctctg
     5161 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5221 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5281 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5341 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5401 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5461 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5521 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5581 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5641 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5701 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     5761 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     5821 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     5881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     5941 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6001 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6061 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6121 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6181 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6241 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6301 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6361 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6421 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6481 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6541 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     6601 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     6661 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7861 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7921 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7981 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8041 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8101 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8161 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8221 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8281 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8341 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8401 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8461 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8521 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8581 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8641 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8701 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8761 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8821 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
     8881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9001 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     9061 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     9121 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
