LOCUS Exported 10,836 bp ds-DNA circular SYN 13-MAY-2019 COMMENT Created by Takara Bio USA, Inc. http://www.takarabio.com COMMENT 631849, 631848, 631847 FEATURES Location/Qualifiers source 1..10836 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR misc_feature 681..806 /label=HIV-1 Psi misc_feature 1303..1536 /label=RRE misc_feature 2028..2143 /label=cPPT/CTS polyA_signal 2187..2321 /label=SV40 poly(A) signal CDS complement(2522..4174) /codon_start=1 /gene="luc" /product="firefly luciferase" promoter complement(4184..4551) /label=TRE3GS promoter promoter 4568..5078 /label=hPGK promoter CDS 5097..5843 /codon_start=1 /label=Tet-On(R) 3G promoter 5854..6183 /label=SV40 promoter rep_origin 6034..6169 /label=SV40 ori CDS 6192..6791 /codon_start=1 /label=PuroR misc_feature 6805..7393 /label=WPRE CDS complement(7276..7287) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site LTR 7600..8233 /label=3' LTR primer_bind complement(8361..8377) /label=M13 rev protein_bind 8385..8401 /label=lac operator promoter complement(8409..8439) /label=lac promoter protein_bind 8454..8475 /label=CAP binding site rep_origin complement(8763..9351) /label=ori CDS complement(9522..10382) /label=AmpR promoter complement(10383..10487) /label=AmpR promoter ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat 2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg 2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg 2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca 2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat 2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg 2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg 2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca 2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg 2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa 2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag 2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga 2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa 2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga 3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct 3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg 3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat 3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa 3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa 3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca 3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg 3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa 3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat 3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa 3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta 3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca 3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc 3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac 3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca 3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca 3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg 4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc 4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagcgga tagaatggcg 4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag 4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc 4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg 4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt 4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa 4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg 4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata 4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg 4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct 4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga 4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt 4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa 4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg 4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc 4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg 5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt 5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta 5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt 5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca 5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca 5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg 5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc 5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac 5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta 5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag 5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa 5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag 5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg 5821 accttgacat gctccccggg taaacgcgcg aatgtgtgtc agttagggtg tggaaagtcc 5881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 5941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 6001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 6061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 6181 aaaacgcgac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 6241 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 6301 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 6361 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 6421 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 6481 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 6541 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 6601 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 6661 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 6721 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 6781 ccggtgcctg aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg 6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 7201 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 7381 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca 7441 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 7501 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 7561 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc 7621 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 7681 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca 7741 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct 7801 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga 7861 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca 7921 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc 7981 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt 8041 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 8101 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 8161 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 8221 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 8281 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc 8341 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 8401 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 8461 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 8521 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 8581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 8641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 8701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 8761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 8821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 8881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 8941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 9001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 9061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 9121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 9181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 9241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 9301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 9361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 9421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 9481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 9541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 9601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 9661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 9721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 9781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 9841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 9901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 9961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 10021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 10081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 10141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 10201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 10261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 10321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 10381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 10441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 10501 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg 10561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 10621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac 10681 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta 10741 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa 10801 ttgtatttgt taaatatgta ctacaaactt agtagt //