LOCUS       Exported               10,836 bp ds-DNA     circular SYN 13-MAY-2019
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT	    631849, 631848, 631847
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
     misc_feature    681..806
                     /label=HIV-1 Psi
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2143
                     /label=cPPT/CTS
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
     CDS             complement(2522..4174)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
     promoter        complement(4184..4551)
                     /label=TRE3GS promoter
     promoter        4568..5078
                     /label=hPGK promoter
     CDS             5097..5843
                     /codon_start=1
                     /label=Tet-On(R) 3G
     promoter        5854..6183
                     /label=SV40 promoter
     rep_origin      6034..6169
                     /label=SV40 ori
     CDS             6192..6791
                     /codon_start=1
                     /label=PuroR
     misc_feature    6805..7393
                     /label=WPRE
     CDS             complement(7276..7287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
     LTR             7600..8233
                     /label=3' LTR
     primer_bind     complement(8361..8377)
                     /label=M13 rev
     protein_bind    8385..8401
                     /label=lac operator
     promoter        complement(8409..8439)
                     /label=lac promoter
     protein_bind    8454..8475
                     /label=CAP binding site
     rep_origin      complement(8763..9351)
                     /label=ori
     CDS             complement(9522..10382)
                     /label=AmpR
     promoter        complement(10383..10487)
                     /label=AmpR promoter

ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
     2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
     2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
     2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
     2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
     2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
     2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
     2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
     2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
     3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
     3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
     3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
     3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
     3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
     3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
     3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
     3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
     3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
     3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
     3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
     3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
     3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
     3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
     3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
     3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
     3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
     4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
     4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagcgga tagaatggcg
     4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag
     4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc
     4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg
     4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt
     4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa
     4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg
     4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata
     4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt
     5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
     5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
     5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5821 accttgacat gctccccggg taaacgcgcg aatgtgtgtc agttagggtg tggaaagtcc
     5881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6181 aaaacgcgac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6241 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6301 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6361 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6421 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6481 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6541 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6601 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6661 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6721 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6781 ccggtgcctg aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     7201 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7381 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     7441 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7501 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7561 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7621 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7681 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7741 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7801 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     7861 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     7921 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     7981 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8041 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8101 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8161 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8221 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8281 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     8341 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8401 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8461 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8521 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8581 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8641 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8701 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8761 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8821 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8881 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8941 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9001 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9061 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9121 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9181 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9241 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9301 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9361 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9421 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9481 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9541 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9601 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9661 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9721 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9781 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9841 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9901 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9961 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10021 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10081 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10141 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10201 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10261 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10321 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10381 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10441 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10501 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    10561 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    10621 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    10681 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    10741 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    10801 ttgtatttgt taaatatgta ctacaaactt agtagt
//
