LOCUS       Exported                9912 bp ds-DNA     circular SYN 13-MAY-2019
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT	    631844, 631845, 631846
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
     misc_feature    681..806
                     /label=HIV-1 Psi
     misc_feature    1303..1536
                     /label=RRE
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
     CDS             complement(2522..4174)
                     /label=luciferase
     promoter        complement(4184..4551)
                     /label=TRE3GS promoter
     promoter        4568..5078
                     /label=hPGK promoter
     CDS             5097..5843
                     /codon_start=1
		     /label=Tet-On 3G
     misc_feature    5857..6445
                     /label=WPRE
     CDS             complement(6328..6339)
                     /codon_start=1
     LTR             6652..7285
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7413..7429)
                     /label=M13 rev
     protein_bind    7437..7453
                     /label=lac operator
     promoter        complement(7461..7491)
                     /label=lac promoter
     protein_bind    7506..7527
                     /label=CAP binding site
     rep_origin      complement(7815..8403)
                     /direction=LEFT
                     /label=ori
     CDS             complement(8574..9434)
                     /product="beta-lactamase"
                     /label=AmpR
     promoter        complement(9435..9539)
                     /label=AmpR promoter
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
     2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
     2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
     2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
     2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
     2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
     2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
     2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
     2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
     3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
     3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
     3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
     3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
     3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
     3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
     3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
     3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
     3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
     3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
     3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
     3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
     3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
     3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
     3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
     3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
     3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
     4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
     4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctctagcgga tagaatggcg
     4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag
     4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc
     4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg
     4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt
     4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa
     4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg
     4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata
     4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt
     5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
     5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
     5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     5821 accttgacat gctccccggg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt
     5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6421 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     6481 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     6541 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     6601 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     6661 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     6721 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     6781 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     6841 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     6901 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     6961 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     7021 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     7081 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7141 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7201 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7261 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7321 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta gcgtttaccg
     7381 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7441 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7501 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7561 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7621 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7681 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7741 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7801 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7861 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7921 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7981 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8041 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8101 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8161 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8221 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8281 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8341 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8401 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8461 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8521 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8581 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8641 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8701 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8761 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8821 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8881 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8941 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9001 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9061 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9121 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9181 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9241 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9301 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9361 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9421 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9481 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9541 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc
     9601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     9721 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     9781 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     9841 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagtaa tatgtactac
     9901 aaacttagta gt
//
