LOCUS       pLVX-Puro   8102 bp    DNA   circular            27-JUL-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632164,632159
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=cPPT/CTS
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4023..4614
                     /label=WPRE
     rep_origin      complement(5923..6596)
                     /label=pUC\origin
     CDS             complement(6741..7737)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             4817..5453
                     /label=3'\LTR
     promoter        2881..3389
                     /label=PGK\Promoter
     CDS             3410..4009
                     /label=Puro(R)
     misc_feature    2815..2879
                     /label=MCS
BASE COUNT     2031 a      2044 c      2115 g      1912 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat 
     2881 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag 
     2941 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca 
     3001 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc 
     3061 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg 
     3121 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg 
     3181 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc 
     3241 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc 
     3301 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt 
     3361 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta 
     3421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 
     3481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 
     3541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 
     3601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 
     3661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 
     3721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 
     3781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 
     3841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 
     3901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 
     3961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga 
     4021 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 
     4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 
     4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 
     4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 
     4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 
     4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 
     4381 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 
     4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 
     4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 
     4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta 
     4621 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 
     4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 
     4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 
     4801 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga 
     4861 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc 
     4921 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga 
     4981 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca 
     5041 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt 
     5101 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg 
     5161 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga 
     5221 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct 
     5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 
     5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     5521 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg 
     5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 
     7741 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     7861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc 
     7921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta 
     7981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac 
     8041 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta 
     8101 gt 
//
