LOCUS       pLVX-PTunerC   8385 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631074,631075
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2031..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     misc_feature    4353..4944
                     /label=WPRE
     rep_origin      complement(6253..6926)
                     /label=pUC\origin
     CDS             complement(7071..8067)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     misc_feature    2031..2046
                     /label=cPPT
     LTR             5147..5783
                     /label=3'\LTR
     misc_feature    3211..3719
                     /label=PGK\promoter
     misc_feature    3740..4339
                     /label=Puro(R)
     misc_feature    2815..2867
                     /label=MCS
     CDS             2880..3200
                     /label=DD-C
BASE COUNT     2095 a      2127 c      2207 g      1956 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 
     2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagag 
     2881 gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag cgcggccaga 
     2941 cctgcgtggt gcactacacc gggatgcttg gagatggaaa gaaagttgac tcctcccggg 
     3001 acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga ggctgggaag 
     3061 aaggggttgc ccagatgagt gtgggtcagg gagccaaact gactatatct ccagattatg 
     3121 cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc gtcttcgatg 
     3181 tggagcttct agaactggaa tagtctagat aattctaccg ggtaggggag gcgcttttcc 
     3241 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg 
     3301 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg 
     3361 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc 
     3421 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga 
     3481 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc 
     3541 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca 
     3601 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca 
     3661 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga 
     3721 cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 
     3781 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 
     3841 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 
     3901 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 
     3961 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 
     4021 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 
     4081 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 
     4141 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 
     4201 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 
     4261 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 
     4321 ccgcaagccc ggtgcctgac cgcgtctgga acaatcaacc tctggattac aaaatttgtg 
     4381 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 
     4441 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 
     4501 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 
     4561 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 
     4621 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 
     4681 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 
     4741 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 
     4801 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 
     4861 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 
     4921 ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag aaaaacatgg 
     4981 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 
     5041 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 
     5101 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat 
     5161 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 
     5221 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg 
     5281 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa 
     5341 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt 
     5401 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga 
     5461 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc 
     5521 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc 
     5581 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     5641 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     5701 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     5761 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     5821 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg 
     5881 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     5941 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     6001 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     6061 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     6121 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     6181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     6241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     6301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     6361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     6421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     6481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     6541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     6601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     6661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     6721 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     6781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     6841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     6901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     6961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     7021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     7081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     7141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     7201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     7261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     7321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     7381 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     7441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     7501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     7561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     7621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     7681 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     7741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     7801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     7861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     7921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     7981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     8041 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc 
     8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 
     8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa 
     8221 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac 
     8281 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 
     8341 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt 
//
