LOCUS       pLVX-Het-Mem1          9220 bp DNA     circular SYN 13-SEP-2021
COMMENT     Created by Takara Bio USA, Inc. http://www.takarabio.com
            Cat. Nos. 635074, 635075
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="5' LTR"
     misc_feature    681..806
                     /label="HIV-1 Psi"
     misc_feature    1303..1536
                     /label="RRE"
     misc_feature    2028..2143
                     /label="cPPT/CTS"
     enhancer        2201..2504
                     /label="CMV enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
     CDS             2821..2862
                     /label="myr"
                     /note="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
     CDS             2869..3189
                     /label="FKBP (DmrA)"
     CDS             3196..3516
                     /label="FKBP (DmrA)"
     misc_feature    3544..4117
                     /label="IRES"
     CDS             4156..5175
                     /label="HygR"
     misc_feature    5189..5777
                     /label="WPRE"
     LTR             5984..6617
                     /label="3' LTR"
     rep_origin      complement(7147..7735)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7906..8766)
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(8767..8871)
                     /label="AmpR promoter"
     polyA_signal    8919..9053
                     /label="SV40 poly(A) signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 atggggagta gcaagagcaa gcctaaggac cccagccagc gctctagagg agtgcaggtg
     2881 gaaaccatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac ctgcgtggtg
     2941 cactacaccg ggatgcttga agatggaaag aaatttgatt cctcccggga cagaaacaag
     3001 ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga aggggttgcc
     3061 cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc ctatggtgcc
     3121 actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt ggagcttcta
     3181 aaactggaaa ctagaggcgt gcaggtcgag accatcagcc ccggcgacgg ccgcaccttt
     3241 cccaagagag gccagacttg cgtggtccac tacaccggca tgctggagga cggcaagaag
     3301 ttcgacagca gccgcgaccg caacaagccc ttcaagttca tgctgggcaa acaggaagtg
     3361 atccgcggct gggaggaagg cgtggctcag atgagcgtgg ggcagcgggc caagctgacc
     3421 atcagccccg actatgccta cggcgccacc ggccaccccg gcatcatccc cccccacgcc
     3481 accctggtgt tcgacgtgga gctgctgaag ctggagagcg gccgcggatc ctacgtatga
     3541 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     3601 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     3661 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     3721 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     3781 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     3841 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     3901 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     3961 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4021 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4081 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cgtaccaaag
     4141 atggatagat ccggaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     4201 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     4261 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     4321 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     4381 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     4441 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     4501 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4561 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4621 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     4681 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     4741 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     4801 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     4861 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     4921 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     4981 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5041 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     5101 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     5161 agggcaaagg aatagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     5221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5581 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5761 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     5821 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5881 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     5941 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     6001 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     6061 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     6121 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     6181 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     6241 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     6301 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     6361 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     6421 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6601 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6661 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc
     6721 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8881 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata
     8941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     9061 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     9121 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     9181 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
