LOCUS       pLVX-EF1a-Tet3G Vector              9851 bp ds-DNA     circular SYN 05-MAR-2020
COMMENT    Created by Takara Bio USA, Inc. http://www.takarabio.com
COMMENT    631359, 631352, 631353, 631354

REFERENCE   1  (bases 1 to 9851)

FEATURES             Location/Qualifiers
     source          1..9851
     LTR             1..635
                     /label=5' LTR
     misc_feature    685..822
                     /label=psi
     misc_feature    1303..1536
                     /label=RRE
     misc_feature    2028..2151
                     /label=cPPT/CTS
                     sequence"
     promoter        2235..3636
                     /label=pEF1-alpha
     CDS             3670..4416
                     /label=Tet-On(R) 3G
     misc_feature    4425..4998
                     /label=IRES
     CDS             5012..5806
                     /label=Neo(R)
     misc_feature    5820..6411
                     /label=WPRE
     LTR             6614..7250
                     /label=3' LTR
     misc_feature    complement(7719..8392)
                     /label=pUC ori
     CDS             complement(8537..9397)
                     /label=AmpR
     promoter        complement(9398..9502)
                     /label=AmpR promoter
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgctcgagga gcttggccca ttgcatacgt tgtatccata
     2221 tcataatatg tacagagtaa ttcatacaaa aggactcgcc cctgccttgg ggaatcccag
     2281 ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc ccgccccctc
     2341 acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct ccggtgcccg
     2401 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2461 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2521 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2581 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2641 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacgcccct
     2701 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     2761 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cttgggcgct
     2821 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     2881 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     2941 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     3001 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     3061 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     3121 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3181 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     3241 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     3301 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     3361 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3421 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     3481 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     3541 gtttttttct tccatttcag gtgtcgtgag agctcgttta gtgaaccgtc agatcgcctg
     3601 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccaaattctc
     3661 gaggccacca tgtctagact ggacaagagc aaagtcataa actctgctct ggaattactc
     3721 aatggagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct gggagttgag
     3781 cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc
     3841 gagatgctgg acaggcatca tacccactcc tgccccctgg aaggcgagtc atggcaagac
     3901 tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg cgacggggct
     3961 aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga aaatcagctc
     4021 gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct gtccgccgtg
     4081 ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc aaaagaggaa
     4141 agagagacac ctaccaccga ttctatgccc ccacttctga aacaagcaat tgagctgttc
     4201 gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat atgtggcctg
     4261 gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta
     4321 gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct
     4381 cttgacgatt ttgaccttga catgctcccc gggtaaggat cccgcccctc tccctccccc
     4441 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     4501 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     4561 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4621 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4681 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4741 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4801 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4861 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4921 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4981 tgaaaaacac gatgataagc ttgccacaac catggctgaa caagatggat tgcacgcagg
     5041 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5101 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5161 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5221 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     5281 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     5341 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     5401 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     5461 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     5521 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     5581 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     5641 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     5701 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     5761 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaacgc gtctggaaca
     5821 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5881 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5941 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6001 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6061 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6121 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6181 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6241 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6301 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6361 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     6421 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6481 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6541 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6601 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6661 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     6721 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     6781 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     6841 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     6901 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     6961 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     7021 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     7081 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7141 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7201 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7261 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7321 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7381 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7441 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7621 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7681 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7741 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7801 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7861 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7921 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7981 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8041 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8101 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8161 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8221 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8281 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8341 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8401 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8461 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8521 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8581 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8641 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8701 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8761 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8821 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8881 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8941 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9001 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9061 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9121 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9181 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9241 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9301 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9361 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9421 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9481 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9541 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9601 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9661 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     9721 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     9781 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     9841 aacttagtag t
//
