LOCUS       pLAPSN   7868 bp    DNA   circular             8-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631508,631459
FEATURES             Location/Qualifiers
     LTR             1..589
                     /label=5'\MMSV\LTR
     misc_signal     659..1468
                     /label=Extended\packaging\signal
     CDS             1579..3168
                     /label=Alkaline\Phosphatase
     CDS             complement(6632..7489)
                     /label=Amp(R)
     rep_origin      5869..5869
                     /label=pUC\origin
     promoter        3488..3840
                     /label=SV40\early\Promoter
     CDS             3886..4680
                     /label=Neo(R)
     LTR             4740..5333
                     /label=3'\MMLV\LTR
BASE COUNT     1732 a      2288 c      2114 g      1734 t 
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 
     1441 tccagccctc actccttctc taggcgccgg aattcgttaa ctcgacccga gctcggatcc 
     1501 actagtaacg gccgccagtg tgctggaatt cctgcctcgc cactgtcctg ctgccctcca 
     1561 gacatgctgg ggccctgcat gctgctgctg ctgctgctgc tgggcctgag gctacagctc 
     1621 tccctgggca tcatcccagt tgaggaggag aacccggact tctggaaccg cgaggcagcc 
     1681 gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa gaacctcatc 
     1741 atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat cctaaaaggg 
     1801 cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt cccatatgtg 
     1861 gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc cacagccacg 
     1921 gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc agccgcccgc 
     1981 tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag 
     2041 aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc ctcgccagcc 
     2101 ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg 
     2161 gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat ggacattgac 
     2221 gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga ccctgagtac 
     2281 ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt gcaggaatgg 
     2341 ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc 
     2401 ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat gaaatacgag 
     2461 atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc tgccctgcgc 
     2521 ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg catcgaccat 
     2581 ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc 
     2641 attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt cactgccgac 
     2701 cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat cttcgggctg 
     2761 gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg aaacggtcca 
     2821 ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag cgggagcccc 
     2881 gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg cgaggacgtg 
     2941 gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc 
     3001 atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg cgacctggcg 
     3061 ccccccgccg gcaccaccga cgccgcgcac ccggggcggt ccgtggtccc cgcgttgctt 
     3121 cctctgctgg ccgggaccct gctgctgctg gagacggcca ctgctccctg agtgtcccgt 
     3181 ccctggggct cctgcttccc catcccggag ttctcctgct ccccacctcc tgtcgtcctg 
     3241 cctggcctcc agcccgagtc gtcatccccg gagtccctat acagaggtcc tgccatggaa 
     3301 ccttcccctc cccgtgcgct ctggggactg agcccatgac accaaacctg ccccttggct 
     3361 gctctcggac tccctacccc aaccccaggg actgcaggtt gtgccctgtg gctgcctgca 
     3421 ccccaggaaa ggagggggct caggccatcc agccaccacc tacagcccag tggggtcgag 
     3481 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 
     3541 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     3601 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     3661 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     3721 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     3781 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgggctgca 
     3841 ggtcgaggcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 
     3901 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 
     3961 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 
     4021 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 
     4081 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 
     4141 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 
     4201 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 
     4261 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 
     4321 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 
     4381 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 
     4441 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 
     4501 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 
     4561 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 
     4621 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 
     4681 gcgggactct ggggttcgat aaaataaaag attttattta gtctccagaa aaagggggga 
     4741 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca 
     4801 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca 
     4861 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca 
     4921 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 
     4981 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta 
     5041 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt 
     5101 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa 
     5161 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg 
     5221 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc 
     5281 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc 
     5341 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct 
     5401 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
     5461 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
     5521 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 
     5581 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 
     5641 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 
     5701 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
     5761 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     5821 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     5881 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     5941 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     6001 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     6061 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     6121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     6181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     6241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     6301 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     6361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     6421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     6481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     6541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     6601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     6661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     6721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     6781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     6841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     6901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 
     6961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     7021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     7081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     7141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     7201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 
     7261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     7321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     7381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     7441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     7501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     7561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 
     7621 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 
     7681 cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc ataccagatc 
     7741 accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg gcttctgcct 
     7801 cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg cccttccgtt 
     7861 tctttgct 
//
