LOCUS       pIRES2-AcGFP1   5307 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632540
FEATURES             Location/Qualifiers
     CDS             3203..3997
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     polyA_signal    2126..2176
                     /label=SV40\early\polyA
                     /note="SV40 early mRNA polyadenylation signal Polyadenylation signals: 1552-1557 & 1581-1586; mRNA 3' ends: 1590 & 1602"
     polyA_signal    4233..4251
                     /label=HSV\TK\polyA
                     /note="Herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signal Polyadenylation signals: 3659-3664 & 3672-3677"
     promoter        2852..3081
                     /label=SV40\early\Promoter
                     /note="SV40 early promoter Enhancer (72-bp tandem repeats): 2278-2349 & 2350-2421; 21-bp repeats: 2425-2445, 2446-2466, & 2468-2488; Early promoter element: 2501-2507; Major transcription start points: 2497, 2535, 2541, & 2546"
     promoter        2740..2775
                     /label=Kan(R)\Promoter
                     /note="Bacterial promoter for expression of Kan R gene; -35 region: 2166-2171; -10 region: 2189-2194; Transcription start point: 2201"
     rep_origin      complement(2223..2678)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      3019..3154
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      4582..5225
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     misc_signal     591..665
                     /label=MCS
                     /note="Multiple Cloning Site"
     misc_feature    666..1250
                     /label=IRES2
     CDS             1254..1970
                     /label=AcGFP1
BASE COUNT     1275 a      1425 c      1388 g      1219 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 
      721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 
      781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 
      841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 
      901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 
      961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 
     1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 
     1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 
     1141 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 
     1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga 
     1261 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg 
     1321 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc 
     1381 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtga 
     1441 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg 
     1501 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg 
     1561 atgacggcaa ctacaagtcg cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc 
     1621 gcatcgagct gaccggcacc gatttcaagg aggatggcaa catcctgggc aataagatgg 
     1681 agtacaacta caacgcccac aatgtgtaca tcatgaccga caaggccaag aatggcatca 
     1741 aggtgaactt caagatccgc cacaacatcg aggatggcag cgtgcagctg gccgaccact 
     1801 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt 
     1861 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacttcg 
     1921 gcttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag tgagcggccg 
     1981 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 
     2041 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 
     2101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     2161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 
     2221 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
     2281 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
     2341 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
     2401 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
     2461 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
     2521 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
     2581 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
     2641 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 
     2701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     2761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 
     2821 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 
     2881 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 
     2941 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 
     3001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     3061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     3121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 
     3181 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3241 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     3301 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     3361 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 
     3421 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     3481 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     3541 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3601 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3661 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3721 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     3781 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     3841 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     3901 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     3961 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     4021 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     4081 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     4141 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 
     4201 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 
     4261 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 
     4321 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 
     4381 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 
     4441 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     4501 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     4561 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     4621 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     4681 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     4741 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     4801 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     4861 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     4921 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     4981 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     5041 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     5101 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     5161 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     5221 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     5281 gtggataacc gtattaccgc catgcat 
//
