LOCUS       pHet-Nuc1   5410 bp    DNA   circular             9-FEB-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 635063,635067
FEATURES             Location/Qualifiers
     CDS             754..1080
                     /label=DmrA
     misc_feature    673..711
                     /label=HA\Tag
     misc_feature    733..753
                     /label=NLS
     promoter        19..613
                     /label=CMV\Promoter
     5'UTR           615..655
                     /label=5'\UTR\(HSV\TK)
     3'UTR           1099..2260
                     /label=3'\UTR\(Rabbit\Beta-globin\intron\plus\polyA)
     promoter        complement(2269..2464)
                     /label=SV40\promoter
     rep_origin      complement(2347..2424)
                     /label=SV40\origin
     rep_origin      complement(2686..3305)
                     /label=pUC\origin
     CDS             complement(3460..4332)
                     /label=Amp(R)
     rep_origin      complement(4901..5207)
                     /label=f1\origin
BASE COUNT     1362 a      1289 c      1325 g      1434 t 
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt 
      661 ccagaagcgc gtatggcttc tagctatcct tatgacgtgc ctgactatgc cagcctggga 
      721 ggaccttcta gtcctaagaa gaagagaaag gtgtctagag gagtgcaggt ggaaaccatc 
      781 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc 
      841 gggatgcttg aagatggaaa gaaatttgat tcctcccggg acagaaacaa gccctttaag 
      901 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt 
      961 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac 
     1021 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa 
     1081 actagttatt gaggatcctg agaacttcag ggtgagtttg gggacccttg attgttcttt 
     1141 ctttttcgct attgtaaaat tcatgttata tggagggggc aaagttttca gggtgttgtt 
     1201 tagaatggga agatgtccct tgtatcacca tggaccctca tgataatttt gtttctttca 
     1261 ctttctactc tgttgacaac cattgtctcc tcttattttc ttttcatttt ctgtaacttt 
     1321 ttcgttaaac tttagcttgc atttgtaacg aatttttaaa ttcacttttg tttatttgtc 
     1381 agattgtaag tactttctct aatcactttt ttttcaaggc aatcagggta tattatattg 
     1441 tacttcagca cagttttaga gaacaattgt tataattaaa tgataaggta gaatatttct 
     1501 gcatataaat tctggctggc gtggaaatat tcttattggt agaaacaact acatcctggt 
     1561 catcatcctg cctttctctt tatggttaca atgatataca ctgtttgaga tgaggataaa 
     1621 atactctgag tccaaaccgg gcccctctgc taaccatgtt catgccttct tctttttcct 
     1681 acagctcctg ggcaacgtgc tggttgttgt gctgtctcat cattttggca aaggattcac 
     1741 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca 
     1801 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 
     1861 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 
     1921 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa 
     1981 tgagtatttg gtttagagtt tggcaacata tgccatatgc tggctgccat gaacaaaggt 
     2041 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata 
     2101 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt 
     2161 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac 
     2221 tactcccagt catagctgtc cctcttctct tatgaagatc cctcgaggag ctttttgcaa 
     2281 aagccctagg cctccaaaaa agcctcttca ctacttctgg aatagctcag aggccgaggc 
     2341 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga 
     2401 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta 
     2461 attgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
     2521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     2581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     2641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     2701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     2761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     2821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     2881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     2941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     3001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     3061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     3121 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     3181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     3241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     3301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     3361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     3421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     3481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     3541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     3601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     3661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     3721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     3781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     3841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     3901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     3961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     4021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     4081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattgcca cctccacctg 
     4141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     4201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     4261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     4321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     4381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     4441 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 
     4501 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     4561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     4621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     4681 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
     4741 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc 
     4801 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 
     4861 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 
     4921 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 
     4981 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 
     5041 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 
     5101 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 
     5161 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 
     5221 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 
     5281 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 
     5341 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac 
     5401 tcactatagg 
//
