LOCUS       pHet-Mem1   5726 bp    DNA   circular             9-FEB-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 635063,635067
FEATURES             Location/Qualifiers
     CDS             1040..1363
                     /label=DmrA
     CDS             710..1036
                     /label=DmrA
     misc_feature    1370..1396
                     /label=HA\tag
     misc_feature    668..709
                     /label=N-myr\signal
     5'UTR           615..655
                     /label=5'\UTR\(HSV\TK)
     promoter        19..613
                     /label=CMV\Promoter
     3'UTR           1415..2576
                     /label=3'\UTR\(Rabbit\beta-globin\intron\plus\polyA)
     promoter        complement(2585..2780)
                     /label=SV40\promoter
     rep_origin      complement(2663..2740)
                     /label=SV40\origin
     rep_origin      complement(3002..3621)
                     /label=pUC\origin
     CDS             complement(3776..4648)
                     /label=Amp(R)
     rep_origin      complement(5217..5523)
                     /label=f1\origin
BASE COUNT     1434 a      1400 c      1427 g      1465 t 
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt 
      661 cgcgcgtatg gggagtagca agagcaagcc taaggacccc agccagcgct ctagaggagt 
      721 gcaggtggaa accatctccc caggagacgg gcgcaccttc cccaagcgcg gccagacctg 
      781 cgtggtgcac tacaccggga tgcttgaaga tggaaagaaa tttgattcct cccgggacag 
      841 aaacaagccc tttaagttta tgctaggcaa gcaggaggtg atccgaggct gggaagaagg 
      901 ggttgcccag atgagtgtgg gtcagagagc caaactgact atatctccag attatgccta 
      961 tggtgccact gggcacccag gcatcatccc accacatgcc actctcgtct tcgatgtgga 
     1021 gcttctaaaa ctggaaacta gaggcgtgca ggtcgagacc atcagccccg gcgacggccg 
     1081 cacctttccc aagagaggcc agacttgcgt ggtccactac accggcatgc tggaggacgg 
     1141 caagaagttc gacagcagcc gcgaccgcaa caagcccttc aagttcatgc tgggcaaaca 
     1201 ggaagtgatc cgcggctggg aggaaggcgt ggctcagatg agcgtggggc agcgggccaa 
     1261 gctgaccatc agccccgact atgcctacgg cgccaccggc caccccggca tcatcccccc 
     1321 ccacgccacc ctggtgttcg acgtggagct gctgaagctg gagactagtt atccgtacga 
     1381 cgtaccagac tacgcataag aaaagtgagg atcctgagaa cttcagggtg agtttgggga 
     1441 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag 
     1501 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat 
     1561 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt 
     1621 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca 
     1681 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc 
     1741 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat 
     1801 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa 
     1861 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt 
     1921 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg 
     1981 ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt 
     2041 ttggcaaagg attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg 
     2101 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg 
     2161 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg 
     2221 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc 
     2281 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc 
     2341 tgccatgaac aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 
     2401 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 
     2461 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 
     2521 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg aagatccctc 
     2581 gaggagcttt ttgcaaaagc cctaggcctc caaaaaagcc tcttcactac ttctggaata 
     2641 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg 
     2701 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga 
     2761 ctatggttgc tgactaattg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     2821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     2881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     2941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     3001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     3061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     3121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     3181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     3241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     3301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     3361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     3421 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     3481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     3541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     3601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     3661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     3721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     3781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     3841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     3901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     3961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     4021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     4081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     4141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     4201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     4261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     4321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     4381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     4441 ttgccacctc cacctgaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     4801 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 
     4861 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     4921 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     4981 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 
     5041 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taaacgttaa 
     5101 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 
     5161 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 
     5221 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 
     5281 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 
     5341 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 
     5401 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 
     5461 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 
     5521 tgcgccgcta cagggcgcgt cgcgccattc gccattcagg ctgcgcaact gttgggaagg 
     5581 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 
     5641 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 
     5701 tgaattgtaa tacgactcac tatagg 
//
