LOCUS       pHet-1   5329 bp    DNA   circular             9-FEB-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 635063,635067
FEATURES             Location/Qualifiers
     CDS             676..966
                     /label=DmrC
     promoter        19..613
                     /label=CMV\Promoter
     5'UTR           615..655
                     /label=5'\UTR\(HSV\TK)
     misc_feature    973..999
                     /label=HA\tag
     3'UTR           1018..2179
                     /label=3'\UTR\(Rabbit\beta-globin\intron\plus\polyA)
     promoter        complement(2188..2383)
                     /label=SV40\promoter
     rep_origin      complement(2266..2343)
                     /label=SV40\origin
     rep_origin      complement(2605..3224)
                     /label=pUC\origin
     CDS             complement(3379..4251)
                     /label=Amp(R)
     rep_origin      complement(4820..5126)
                     /label=f1\origin
BASE COUNT     1348 a      1259 c      1301 g      1421 t 
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt 
      661 ccagaagccg ccaccatggc ttctagaatc ctctggcatg agatgtggca tgaaggcctg 
      721 gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg 
      781 gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat 
      841 caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca 
      901 gggaatgtca aggacctcct ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc 
      961 tcaaagacta gttatccgta cgacgtacca gactacgcat aagaaaagtg aggatcctga 
     1021 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt 
     1081 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt 
     1141 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc 
     1201 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca 
     1261 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta 
     1321 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag 
     1381 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg 
     1441 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt 
     1501 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg 
     1561 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct 
     1621 ggttgttgtg ctgtctcatc attttggcaa aggattcact cctcaggtgc aggctgccta 
     1681 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt 
     1741 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 
     1801 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 
     1861 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 
     1921 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt 
     1981 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt 
     2041 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct 
     2101 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc 
     2161 ctcttctctt atgaagatcc ctcgaggagc tttttgcaaa agccctaggc ctccaaaaaa 
     2221 gcctcttcac tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat 
     2281 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg 
     2341 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgctgcatt aatgaatcgg 
     2401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     2461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     2521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     2581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     2641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     2701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     2761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     2821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     2881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     2941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     3001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     3061 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     3121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     3181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     3241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     3301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     3361 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     3421 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     3481 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     3541 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     3601 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     3661 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     3721 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     3781 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     3841 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     3901 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     3961 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     4021 cagaacttta aaagtgctca tcattgccac ctccacctga aaacgttctt cggggcgaaa 
     4081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     4141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     4201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     4261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     4321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     4381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 
     4441 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 
     4501 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 
     4561 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 
     4621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 
     4681 catcaggaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 
     4741 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 
     4801 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 
     4861 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 
     4921 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 
     4981 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 
     5041 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 
     5101 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcgcgcca ttcgccattc 
     5161 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 
     5221 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 
     5281 cgacgttgta aaacgacggc cagtgaattg taatacgact cactatagg 
//
