LOCUS       pGuide-it-sgRNA1 Vector (Linear)                3024 bp ds-DNA     linear   SYN 30-OCT-2015
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat No. 632612, 632613, 632614
SOURCE      synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1..93
                     /label="sgRNA"
                     /note="single-guide RNA scaffold segment; user clones guide sequence"
     rep_origin      complement(517..1105)
                     /direction=LEFT
                     /label="ori"
                     /note="ori; high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1276..2136)
                     /label="AmpR"
                     /note="beta-lactamase, confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(2137..2241)
                     /label="AmpR promoter"
     promoter        2761..3016
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
ORIGIN
        1 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
       61 ttgaaaaagt ggcaccgagt cggtgctttt tttctaaagc ttggcgtaat catggtcata
      121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      421 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      721 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      781 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      841 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      901 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      961 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1021 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1081 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1141 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1201 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     1441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     1501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     1561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     1621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     1681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     1741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2281 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2341 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2401 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2461 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2521 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     2581 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2641 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2701 ccagtcacga cgttgtaaaa cgacggccag tgaattccgg tcctaaggta gcgaaagctc
     2761 agatctgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
     2821 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
     2881 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
     2941 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
     3001 atcttgtgga aaggacgaaa cacc
//
