LOCUS       pGBKT7   7303 bp    DNA   circular             6-JAN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630443,630489
FEATURES             Location/Qualifiers
     misc_feature    30..736
                     /label=Truncated\ADH1\Promoter
     misc_feature    762..1202
                     /label=GAL4\DNA\binding\domain
     misc_feature    1212..1235
                     /label=T7\RNA\Polymerase\Promoter
     misc_feature    1248..1280
                     /label=c-Myc\tag
     misc_feature    1281..1334
                     /label=MCS
     misc_feature    1335..1381
                     /label=T7\terminator
     misc_feature    1414..1610
                     /label=ADH1\terminator
     misc_feature    1838..2636
                     /label=pUC\origin
     misc_feature    complement(3221..4143)
                     /label=Kan(R)
     misc_feature    4147..5492
                     /label=2\micron\origin
     misc_feature    6030..6704
                     /label=TRP1
     misc_feature    6755..29
                     /label=f1\origin
BASE COUNT     1977 a      1653 c      1737 g      1936 t 
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac 
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga 
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc 
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt 
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atccgtcgac 
     1321 ctgcagcggc cgcataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 
     1381 ttttgcgcgc ttgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 
     1441 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 
     1501 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 
     1561 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 
     1621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 
     1681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 
     1741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 
     1801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     1861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     1921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 
     2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     2461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     2761 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 
     2821 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 
     2881 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 
     2941 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 
     3001 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag 
     3061 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 
     3121 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 
     3181 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa 
     3241 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc 
     3301 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct 
     3361 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt 
     3421 ccaccatgat attcggcaag caggcatcgc catgagtcac gacgagatcc tcgccgtcgg 
     3481 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt 
     3541 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat 
     3601 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg 
     3661 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc 
     3721 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag 
     3781 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt 
     3841 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca 
     3901 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata 
     3961 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatactct 
     4021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 
     4081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 
     4141 cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta 
     4201 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg 
     4261 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag 
     4321 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga 
     4381 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc 
     4441 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct 
     4501 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta 
     4561 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga 
     4621 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat 
     4681 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt 
     4741 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg 
     4801 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg 
     4861 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt 
     4921 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca 
     4981 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt 
     5041 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa 
     5101 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa 
     5161 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact 
     5221 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc 
     5281 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg 
     5341 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca 
     5401 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc 
     5461 aatgctatca tttcctttga tattggatca tactaagaaa ccattattat catgacatta 
     5521 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt 
     5581 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
     5641 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 
     5701 aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac gacattacta 
     5761 tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt 
     5821 tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta 
     5881 cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa 
     5941 aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg 
     6001 tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg 
     6061 gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt 
     6121 ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg 
     6181 ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga 
     6241 aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg 
     6301 attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt 
     6361 tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca 
     6421 gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga 
     6481 caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg 
     6541 aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta 
     6601 gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag 
     6661 actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt 
     6721 atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat 
     6781 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc 
     6841 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 
     6901 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 
     6961 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 
     7021 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 
     7081 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 
     7141 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 
     7201 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 
     7261 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat 
//
