LOCUS       pGADT7-RecAB   8250 bp    DNA   circular             1-FEB-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630488,630482,630486,630487,630479,630481,630485,630483,630480,630484
FEATURES             Location/Qualifiers
     misc_feature    7..1478
                     /label=ADH1\Promoter
     misc_feature    1500..1556
                     /label=SV40\Nuclear\Localization\Signal\(NLS)
     misc_feature    1560..1898
                     /label=GAL4\AD
     misc_feature    1897..2035
                     /label=MCS\A
     misc_feature    1904..1926
                     /label=T7\RNA\Polymerase\Promoter
     misc_feature    1938..1967
                     /label=Hemagglutination\(HA)\tag
     misc_feature    2000..2035
                     /label=SMART\IV\oligo\sequence
     misc_feature    2036..2243
                     /label=Stuffer\fragment
     misc_feature    2244..2262
                     /label=CDS\III\primer\sequence
     misc_feature    2244..2297
                     /label=MCS\B
     misc_feature    2244..2297
                     /label=ADH1\transcription\terminator
     misc_feature    complement(2982..4076)
                     /label=LEU2
     misc_feature    4843..5680
                     /label=pUC\origin
     misc_feature    complement(5836..6696)
                     /label=Amp(R)
     misc_feature    7260..8250
                     /label=2\micron\origin
BASE COUNT     2364 a      1835 c      1740 g      2311 t 
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag 
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct 
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg 
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat 
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca 
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag 
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca 
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt 
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata 
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atgaacatgg 
     1981 aggccagtga attccaccca agcagtggta tcaacgcaga gtggccatta cggcctgcag 
     2041 gatccgaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt 
     2101 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     2161 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     2221 tgtatcttat catgtctgga tccggccgcc tcggcctcta gagggtgggc atcgatacgg 
     2281 gatccatcga gctcgagctg cagatgaatc gtagatactg aaaaaccccg caagttcact 
     2341 tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt 
     2401 tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt 
     2461 ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata 
     2521 tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc 
     2581 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca 
     2641 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat 
     2701 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta 
     2761 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc 
     2821 tcaggtatag catgaggtcg ctcttattga ccacacctct accggccggt cgaaattccc 
     2881 ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt 
     2941 ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta 
     3001 acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca 
     3061 gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca 
     3121 ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg 
     3181 accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg 
     3241 gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg 
     3301 ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca 
     3361 tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga 
     3421 accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt 
     3481 gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt 
     3541 agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca 
     3601 ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc 
     3661 actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat 
     3721 aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt 
     3781 tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc ccatttagga 
     3841 ccacccacag cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct 
     3901 ggaagtggga cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg 
     3961 aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg 
     4021 atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga 
     4081 atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag 
     4141 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt 
     4201 caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa 
     4261 agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat 
     4321 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg 
     4381 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat 
     4441 tcgaactctt gcatcttacg atacctgagt attcccacag ttgggggatc tcgactctag 
     4501 ctagaggatc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     4561 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     4621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     4681 tcgtgccagc tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg 
     4741 agtttacgtc cagccaagct agcttggctg caggtcgagc ggccgcgatc cggaaccctt 
     4801 aatataactt cgtataatgt atgctatacg aagttatcag ctgcattaat gaatcggcca 
     4861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     4921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     4981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     5041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     5101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     5161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     5221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     5281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     5341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     5401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     5461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 
     5521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     5581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     5641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     5701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     5761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     5821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     5881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     5941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     6001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     6061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     6121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     6181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     6241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     6301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     6361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     6421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     6481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     6541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     6601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     6661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     6721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     6781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
     6841 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 
     6901 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     6961 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     7021 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     7081 taacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg 
     7141 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca 
     7201 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct 
     7261 ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg 
     7321 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac 
     7381 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct 
     7441 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat 
     7501 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca 
     7561 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac 
     7621 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt 
     7681 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat 
     7741 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg 
     7801 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct 
     7861 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa 
     7921 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag 
     7981 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa 
     8041 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa 
     8101 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt 
     8161 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc 
     8221 tacaaaatga agcacagatg cttcgttgct 
//
