LOCUS       pGADT7-Rec   8058 bp    DNA   circular            23-DEC-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630471,630477,630478,630469,630491,630468,630490,630470,630476
FEATURES             Location/Qualifiers
     misc_feature    7..1478
                     /label=ADH1\Promoter
     misc_feature    1500..1556
                     /label=SV40\NLS
     misc_feature    1560..1898
                     /label=GAL4\AD
     misc_feature    1904..1926
                     /label=T7\Promoter
     misc_feature    1941..1967
                     /label=Hemagglutinin\(HA)\tag
     misc_feature    2000..2035
                     /label=SMART\III\Oligonucleotide\Sequence
     misc_feature    2046..2070
                     /label=CDS\III\Sequence
     misc_feature    2350..2675
                     /label=ADH1\Transcription\terminator
     misc_feature    complement(2793..3884)
                     /label=LEU2
     misc_feature    4651..5488
                     /label=pUC\origin
     misc_feature    complement(5645..6502)
                     /label=Amp(R)
     misc_feature    7068..8058
                     /label=2\micron\origin
BASE COUNT     2302 a      1793 c      1716 g      2247 t 
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag 
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct 
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg 
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat 
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca 
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag 
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca 
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt 
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata 
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg 
     1981 aggccagtga attccaccca agcagtggta tcaacgcaga gtggccatta tggcccggga 
     2041 aaaaacatgt cggccgcctc ggcctctaga gggtgggcat cgatacggga tccatcgagc 
     2101 tcgagctgca gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat 
     2161 cgtgcaccat ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata 
     2221 ctcctctaag tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact 
     2281 agaattaatg cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc 
     2341 ttattcagaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt 
     2401 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag 
     2461 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa 
     2521 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg 
     2581 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca 
     2641 tgaggtcgct cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa 
     2701 catattccat tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg 
     2761 tacaaatatc ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg 
     2821 acagcatcac cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct 
     2881 gcatccaaaa cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat 
     2941 gacaatttca acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt 
     3001 ggcaaatctg gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct 
     3061 ggcaaagagg ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct 
     3121 tcatcggaga tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt 
     3181 gggttcttaa ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta 
     3241 ggaaattcgt tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa 
     3301 acattagctt tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa 
     3361 gcggccattc ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg 
     3421 acaccatcac catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg 
     3481 acaacaacga agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga 
     3541 gagtcggatg caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt 
     3601 tttagtaaac cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca 
     3661 cctaacaaaa cggcatcagc cttcttggag gcttccagcg cctcatctgg aagtgggaca 
     3721 cctgtagctt cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg 
     3781 gaacgaacat cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca 
     3841 acgtggtcac ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct 
     3901 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga 
     3961 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta 
     4021 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg 
     4081 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg 
     4141 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc 
     4201 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc 
     4261 atcttacgat acctgagtat tcccacagtt gggggatctc gactctagct agaggatcaa 
     4321 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     4381 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     4501 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca 
     4561 gccaagctag cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg 
     4621 tataatgtat gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag 
     4681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     4741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     4801 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     4861 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     4921 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     4981 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     5041 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     5101 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     5161 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     5221 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     5281 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 
     5341 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     5401 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     5461 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     5521 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     5581 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     5641 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     5701 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     5761 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     5821 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     5881 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     5941 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     6001 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     6061 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     6121 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     6181 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     6241 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     6301 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     6361 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     6421 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     6481 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     6541 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     6601 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 
     6661 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 
     6721 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 
     6781 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 
     6841 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa 
     6901 gcataaacac gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat 
     6961 ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg 
     7021 ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag 
     7081 tataggaact tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa 
     7141 accaaaaacg caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa 
     7201 cattgctcaa aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc 
     7261 catccacctt tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta 
     7321 tctctagtat tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg 
     7381 aaaacaatac gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa 
     7441 accgttcata attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa 
     7501 gtatgcgcaa tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg 
     7561 cacccgcagc ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg 
     7621 aagagaaaat agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa 
     7681 gttatcaaga gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt 
     7741 tgttagaaaa atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc 
     7801 tttgttggta aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat 
     7861 tctttgtttg aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca 
     7921 gattcttcgt tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct 
     7981 cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag 
     8041 cacagatgct tcgttgct 
//
