LOCUS       pGADT7 AD   7987 bp    DNA   circular             9-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 630442,630489,630491
FEATURES             Location/Qualifiers
     misc_feature    7..1478
                     /label=ADH1\Promoter
     misc_feature    1500..1556
                     /label=SV40\Nuclear\Localization\Signal
     misc_feature    1560..1898
                     /label=GAL4\AD
     misc_feature    1904..1926
                     /label=T7\RNA\Polymerase\Promoter
     misc_feature    1941..1967
                     /label=HA\epitope\tag
     misc_feature    1968..2040
                     /label=MCS
     misc_feature    2279..2604
                     /label=ADH1\transcription\termination\signal
     misc_feature    complement(2722..3813)
                     /label=LEU2
     misc_feature    4580..5417
                     /label=pUC\origin
     misc_feature    complement(5573..6433)
                     /label=Amp(R)
     misc_feature    6997..7987
                     /label=Yeast\2\micron\origin
BASE COUNT     2283 a      1774 c      1695 g      2235 t 
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag 
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct 
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg 
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat 
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca 
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag 
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca 
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt 
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata 
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg 
     1981 aggccagtga attccacccg ggtgggcatc gatacgggat ccatcgagct cgagctgcag 
     2041 atgaatcgta gatactgaaa aaccccgcaa gttcacttca actgtgcatc gtgcaccatc 
     2101 tcaatttctt tcatttatac atcgttttgc cttcttttat gtaactatac tcctctaagt 
     2161 ttcaatcttg gccatgtaac ctctgatcta tagaattttt taaatgacta gaattaatgc 
     2221 ccatcttttt tttggaccta aattcttcat gaaaatatat tacgagggct tattcagaag 
     2281 ctttggactt cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta 
     2341 ccttgccaga aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg 
     2401 acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa 
     2461 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat 
     2521 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc 
     2581 ttattgacca cacctctacc ggccggtcga aattccccta ccctatgaac atattccatt 
     2641 ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca 
     2701 taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc 
     2761 gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac 
     2821 ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa 
     2881 catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg 
     2941 agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc 
     3001 caaggacgca gatggcaaca aacccaagga acctgggata acggaggctt catcggagat 
     3061 gatatcacca aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac 
     3121 taggatcatg gcggcagaat caatcaattg atgttgaacc ttcaatgtag gaaattcgtt 
     3181 cttgatggtt tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt 
     3241 atccaaggac caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct 
     3301 tgtgattctt tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc 
     3361 atcgtcttcc tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa 
     3421 gtcagtacct ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc 
     3481 aaagttacat ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc 
     3541 ttgttcaggt ctaacactac ctgtacccca tttaggacca cccacagcac ctaacaaaac 
     3601 ggcatcagcc ttcttggagg cttccagcgc ctcatctgga agtgggacac ctgtagcttc 
     3661 gatagcagca ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc 
     3721 agaaatagct ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc 
     3781 tggcaaaacg acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata 
     3841 tatatattgc tgaaatgtaa aaggtaagaa aagttagaaa gtaagacgat tgctaaccac 
     3901 ctattggaaa aaacaatagg tccttaaata atattgtcaa cttcaagtat tgtgatgcaa 
     3961 gcatttagtc atgaacgctt ctctattcta tatgaaaagc cggttccggc gctctcacct 
     4021 ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat 
     4081 taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc 
     4141 gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata 
     4201 cctgagtatt cccacagttg ggggatctcg actctagcta gaggatcaat tcgtaatcat 
     4261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     4321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     4381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctga taacttcgta 
     4441 taatgtatgc tatacgaagt tattaggtct gaagaggagt ttacgtccag ccaagctagc 
     4501 ttggctgcag gtcgagcggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg 
     4561 ctatacgaag ttatcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     4621 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     4681 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     4741 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     4801 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     4861 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     4921 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     4981 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 
     5041 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     5101 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     5161 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     5221 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 
     5281 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     5341 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     5401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     5461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     5521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     5581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     5641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     5701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     5761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     5821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     5881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     5941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     6001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     6061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     6121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     6181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     6241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     6301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     6361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     6421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     6481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     6541 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 
     6601 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa 
     6661 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 
     6721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact 
     6781 atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg 
     6841 cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac 
     6901 gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg 
     6961 aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt 
     7021 cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc 
     7081 accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa 
     7141 agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt 
     7201 cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt 
     7261 actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg 
     7321 aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa 
     7381 ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat 
     7441 ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct 
     7501 tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata 
     7561 gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag 
     7621 actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa 
     7681 tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa 
     7741 aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga 
     7801 aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt 
     7861 ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg 
     7921 tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt 
     7981 cgttgct 
//
