LOCUS       pEF1a-mCherry-N1   5479 bp    DNA   circular            17-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631969
FEATURES             Location/Qualifiers
     misc_feature    1348..1422
                     /label=MCS
     CDS             3375..4169
                     /label=Kan(R)/Neo(R)
     rep_origin      4754..5397
                     /label=pUC\origin
     rep_origin      complement(2395..2850)
                     /label=f1\origin
     rep_origin      3191..3329
                     /label=SV40\origin
     CDS             1436..2143
                     /label=mCherry
     misc_feature    1429..1439
                     /label=Kozak\sequence
     polyA_signal    4405..4423
                     /label=HSV\TK\polyA\signals
     polyA_signal    2298..2332
                     /label=SV40\polyA\signal
     promoter        12..1346
                     /label=EF1a\Promoter
     promoter        3024..3292
                     /label=SV40\early\Promoter
BASE COUNT     1209 a      1489 c      1555 g      1226 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc tcgagctcaa 
     1381 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt 
     1441 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca 
     1501 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc 
     1561 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc 
     1621 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc 
     1681 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat 
     1741 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga 
     1801 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca 
     1861 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct 
     1921 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt 
     1981 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat 
     2041 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc 
     2101 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtagcggc cgcgactcta 
     2161 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca 
     2221 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc 
     2281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 
     2341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg 
     2401 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     2461 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     2521 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     2581 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     2641 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     2701 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     2761 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     2821 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg 
     2881 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     2941 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca 
     3001 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     3061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     3241 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     3301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat 
     3361 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     3421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     3481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     3541 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     3601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     3661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     3721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     3781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     3841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     3901 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     3961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     4021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     4081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     4141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     4201 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     4261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     4321 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc 
     4381 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc 
     4441 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag 
     4501 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc 
     4561 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg 
     4621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 
     4681 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 
     4741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 
     4801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 
     4861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 
     4921 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 
     4981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 
     5041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 
     5101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 
     5161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 
     5221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 
     5281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 
     5341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 
     5401 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 
     5461 ccgtattacc gccatgcat 
//
