LOCUS       pEF1a-Myc   4622 bp    DNA   circular            16-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631991
FEATURES             Location/Qualifiers
     promoter        26..1360
                     /label=EF1a\Promoter
     rep_origin      complement(2376..3019)
                     /label=pUC\origin
     CDS             3167..4163
                     /label=Amp(R)
     CDS             1660..1698
                     /label=Myc\tag
     misc_feature    1703..1757
                     /label=MCS
     misc_feature    1784..1786
                     /label=Stop
     misc_feature    1774..1776
                     /label=Stop
     misc_feature    1771..1773
                     /label=Stop
BASE COUNT     1048 a      1165 c      1269 g      1140 t 
ORIGIN
        1 gagttcgagc ttgcatgcct gcagggagta attcatacaa aaggactcgc ccctgccttg 
       61 gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga tcgctgcgtt 
      121 cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc 
      181 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 
      241 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg 
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact 
      481 tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg 
      541 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg 
      601 gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc 
      661 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg 
      721 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc 
      781 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 
      841 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 
      901 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 
      961 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 
     1021 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 
     1081 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 
     1141 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 
     1201 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 
     1261 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 
     1321 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gagctcgttt agtgaaccgt 
     1381 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga 
     1441 tccagcctcc ggactctaga ggatccggta ctagaggaac tgaaaaacca gaaagttaac 
     1501 tggtaagttt agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc 
     1561 aaagaactgc tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt 
     1621 ctgctctaaa agctgcggaa ttgtacccgc gggcccacca tggcatcaat gcagaagctg 
     1681 atctcagagg aggacctgct tatggccatg gaggcccgaa ttcggtcgac cgagatctct 
     1741 cgaggtaccg cggccgcggg gatccagaca tgataagata cattgatgag tttggacaaa 
     1801 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 
     1861 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta 
     1921 tgtttcaggt tcagggggag gtgtgggagg ttttttcgga tcctctagag tcgatctgca 
     1981 ggcatgctag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 
     2041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 
     2101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 
     2161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 
     2221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 
     2281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     2641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     2701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     2761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     2821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 
     2881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     2941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     3001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     3061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     3121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     3181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     3241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     3301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     3361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     3421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     3481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     3541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     3601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     3661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     3721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     3781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     3841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     3901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     3961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     4021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     4081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     4141 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 
     4201 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 
     4261 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 
     4321 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 
     4381 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 
     4441 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 
     4501 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 
     4561 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 
     4621 gt 
//
