LOCUS       pEF1a-HA   4613 bp    DNA   circular            16-FEB-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631992
FEATURES             Location/Qualifiers
     CDS             1660..1689
                     /label=HA\tag
     promoter        26..1360
                     /label=EF1a\Promoter
     misc_feature    1694..1748
                     /label=MCS
     rep_origin      complement(2367..3010)
                     /label=pUC\origin
     CDS             3158..4154
                     /label=Amp(R)
     misc_feature    1762..1764
                     /label=Stop
     misc_feature    1765..1767
                     /label=Stop
     misc_feature    1775..1777
                     /label=Stop
BASE COUNT     1044 a      1165 c      1262 g      1142 t 
ORIGIN
        1 gagttcgagc ttgcatgcct gcagggagta attcatacaa aaggactcgc ccctgccttg 
       61 gggaatccca gggaccgtcg ttaaactccc actaacgtag aacccagaga tcgctgcgtt 
      121 cccgccccct cacccgcccg ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc 
      181 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 
      241 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg 
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact 
      481 tccacgcccc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg 
      541 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg 
      601 gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc 
      661 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg 
      721 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc 
      781 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 
      841 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 
      901 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 
      961 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 
     1021 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 
     1081 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 
     1141 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 
     1201 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 
     1261 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 
     1321 agtggttcaa agtttttttc ttccatttca ggtgtcgtga gagctcgttt agtgaaccgt 
     1381 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga 
     1441 tccagcctcc ggactctaga ggatccggta ctagaggaac tgaaaaacca gaaagttaac 
     1501 tggtaagttt agtctttttg tcttttattt caggtcccgg atccggtggt ggtgcaaatc 
     1561 aaagaactgc tcctcagtgg atgttgcctt tacttctagg cctgtacgga agtgttactt 
     1621 ctgctctaaa agctgcggaa ttgtacccgc gggcccacca tgtacccata cgatgttcca 
     1681 gattacgctc ttatggccat ggaggcccga attcggtcga ccgagatctc tcgaggtacc 
     1741 gcggccgcgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta 
     1801 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa 
     1861 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg 
     1921 ttcaggggga ggtgtgggag gttttttcgg atcctctaga gtcgatctgc aggcatgcta 
     1981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     2041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     2101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     2221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     2281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     2341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     2581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     2761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     2821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     2881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     2941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     3001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     3061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     3121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     3181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     3241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     3301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     3361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     3421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     3481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     3541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     3601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     3661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     3721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     3781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     3841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     3901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     3961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     4021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     4081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     4141 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 
     4201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 
     4261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 
     4321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 
     4381 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     4441 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 
     4501 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 
     4561 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agt 
//
