LOCUS       pEF1a-E2-Crimson   5395 bp    DNA   circular            17-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631981
FEATURES             Location/Qualifiers
     CDS             3291..4085
                     /label=Kan(R)/Neo(R)
     rep_origin      4670..5313
                     /label=pUC\origin
     polyA_signal    4321..4339
                     /label=HSV\TK\polyA\signal
     CDS             1385..2062
                     /label=E2\Crimson
     misc_signal     1378..1388
                     /label=Kozak\sequence
     promoter        12..1346
                     /label=EF1a\Promoter
     polyA_signal    2214..2248
                     /label=SV40\polyA\signal
     rep_origin      complement(2311..2766)
                     /label=f1\origin
     rep_origin      3107..3245
                     /label=SV40\origin
     misc_feature    2940..3208
                     /label=SV40\early\Promoter
BASE COUNT     1183 a      1478 c      1513 g      1221 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc caccggtcgc 
     1381 caccatggat agcactgaga acgtcatcaa gcccttcatg cgcttcaagg tgcacatgga 
     1441 gggctccgtg aacggccacg agttcgagat cgagggcgtg ggcgagggca agccctacga 
     1501 gggcacccag accgccaagc tgcaagtgac caagggcggc cccctgccct tcgcctggga 
     1561 catcctgtcc ccccagttct tctacggctc caaggcgtac atcaagcacc ccgccgacat 
     1621 ccccgactac ctcaagcagt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 
     1681 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcaccctcat 
     1741 ctaccacgtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa 
     1801 gactctgggc tgggagccct ccactgagcg caactacccc cgcgacggcg tgctgaaggg 
     1861 cgagaaccac atggcgctga agctgaaggg cggcggccac tacctgtgtg agttcaagtc 
     1921 catctacatg gccaagaagc ccgtgaagct gcccggctac cactacgtgg actacaagct 
     1981 cgacatcacc tcccacaacg aggactacac cgtggtggag cagtacgagc gcgccgaggc 
     2041 ccgccaccac ctgttccagt agcggccgcg actctagatc ataatcagcc ataccacatt 
     2101 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 
     2161 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 
     2221 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     2281 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat 
     2341 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 
     2401 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca 
     2461 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg 
     2521 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta 
     2581 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg 
     2641 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 
     2701 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg 
     2761 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 
     2821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
     2881 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg 
     2941 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 
     3001 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     3061 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     3121 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     3181 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3241 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac 
     3301 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 
     3361 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 
     3421 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg 
     3481 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 
     3541 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 
     3601 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 
     3661 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 
     3721 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 
     3781 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc 
     3841 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 
     3901 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 
     3961 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 
     4021 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 
     4081 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 
     4141 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 
     4201 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag 
     4261 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc 
     4321 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg 
     4381 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg 
     4441 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc 
     4501 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga 
     4561 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 
     4621 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 
     4681 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 
     4741 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 
     4801 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 
     4861 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 
     4921 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 
     4981 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 
     5041 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 
     5101 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 
     5161 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 
     5221 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 
     5281 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 
     5341 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 
//
