LOCUS       pEF1a-DsRed-Monomer-N1   5448 bp    DNA   circular            17-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631978
FEATURES             Location/Qualifiers
     CDS             3344..4138
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     rep_origin      complement(2364..2819)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      3160..3295
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      4723..5366
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     CDS             1436..2110
                     /label=DsRed\Monomer
     misc_feature    1429..1439
                     /label=Kozak\sequence
     promoter        12..1346
                     /label=EF1a\Promoter
     polyA_signal    2267..2301
                     /label=SV40\polyA\signal
     polyA_signal    4374..4392
                     /label=HSV\TK\polyA\signals
     misc_feature    1348..1422
                     /label=MCS
     promoter        2993..3261
                     /label=SV40\early\Promoter
BASE COUNT     1198 a      1493 c      1537 g      1220 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc tcgagctcaa 
     1381 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgga 
     1441 caacaccgag gacgtcatca aggagttcat gcagttcaag gtgcgcatgg agggctccgt 
     1501 gaacggccac tacttcgaga tcgagggcga gggcgagggc aagccctacg agggcaccca 
     1561 gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc 
     1621 cccccagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta 
     1681 catgaagctg tccttccccg agggcttcac ctgggagcgc tccatgaact tcgaggacgg 
     1741 cggcgtggtg gaggtgcagc aggactcctc cctgcaggac ggcaccttca tctacaaggt 
     1801 gaagttcaag ggcgtgaact tccccgccga cggccccgta atgcagaaga agactgccgg 
     1861 ctgggagccc tccaccgaga agctgtaccc ccaggacggc gtgctgaagg gcgagatctc 
     1921 ccacgccctg aagctgaagg acggcggcca ctacacctgc gacttcaaga ccgtgtacaa 
     1981 ggccaagaag cccgtgcagc tgcccggcaa ccactacgtg gactccaagc tggacatcac 
     2041 caaccacaac gaggactaca ccgtggtgga gcagtacgag cacgccgagg cccgccactc 
     2101 cggctcccag tagagcggcc gcgactctag atcataatca gccataccac atttgtagag 
     2161 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 
     2221 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     2281 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     2341 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 
     2401 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 
     2461 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 
     2521 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 
     2581 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 
     2641 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 
     2701 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 
     2761 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 
     2821 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     2881 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 
     2941 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 
     3001 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 
     3061 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 
     3121 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 
     3181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
     3241 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
     3301 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 
     3361 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 
     3421 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 
     3481 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 
     3541 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 
     3601 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 
     3661 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 
     3721 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 
     3781 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 
     3841 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 
     3901 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 
     3961 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 
     4021 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 
     4081 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 
     4141 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 
     4201 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 
     4261 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 
     4321 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 
     4381 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 
     4441 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 
     4501 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 
     4561 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 
     4621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     4681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     4741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     4801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     4861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 
     4921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     4981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     5041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     5101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     5161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     5221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     5281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     5341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
     5401 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat 
//
