LOCUS       pEF1a-DsRed-Monomer-C1   5446 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631977
FEATURES             Location/Qualifiers
     rep_origin      3158..3296
                     /label=SV40\origin
     CDS             3342..4136
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     rep_origin      4721..5364
                     /label=pUC\origin
     rep_origin      complement(2362..2817)
                     /label=f1\origin
     CDS             1370..2044
                     /label=DsRed\Monomer
     misc_feature    1363..1373
                     /label=Kozak\sequence
     promoter        12..1346
                     /label=EF1a\Promoter
     polyA_signal    2265..2299
                     /label=SV40\polyA\signal
     polyA_signal    4372..4390
                     /label=HSV\TK\polyA\signals
     misc_feature    2045..2110
                     /label=MCS
     promoter        2991..3259
                     /label=SV40\early\Promoter
     misc_feature    2119..2121
                     /label=Stop
     misc_feature    2123..2125
                     /label=Stop
     misc_feature    2127..2129
                     /label=Stop
BASE COUNT     1199 a      1492 c      1533 g      1222 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gtcgccacca tggacaacac 
     1381 cgaggacgtc atcaaggagt tcatgcagtt caaggtgcgc atggagggct ccgtgaacgg 
     1441 ccactacttc gagatcgagg gcgagggcga gggcaagccc tacgagggca cccagaccgc 
     1501 caagctgcag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca 
     1561 gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacatgaa 
     1621 gctgtccttc cccgagggct tcacctggga gcgctccatg aacttcgagg acggcggcgt 
     1681 ggtggaggtg cagcaggact cctccctgca ggacggcacc ttcatctaca aggtgaagtt 
     1741 caagggcgtg aacttccccg ccgacggccc cgtaatgcag aagaagactg ccggctggga 
     1801 gccctccacc gagaagctgt acccccagga cggcgtgctg aagggcgaga tctcccacgc 
     1861 cctgaagctg aaggacggcg gccactacac ctgcgacttc aagaccgtgt acaaggccaa 
     1921 gaagcccgtg cagctgcccg gcaaccacta cgtggactcc aagctggaca tcaccaacca 
     1981 caacgaggac tacaccgtgg tggagcagta cgagcacgcc gaggcccgcc actccggctc 
     2041 ccagtccgga ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg 
     2101 cccgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt 
     2161 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
     2221 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     2281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     2341 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     2401 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     2461 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     2521 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     2581 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     2641 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     2701 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     2761 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 
     2821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     2941 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 
     3001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     3121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     3181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     3241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     3301 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 
     3361 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 
     3421 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 
     3481 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc 
     3541 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 
     3601 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 
     3661 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 
     3721 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 
     3781 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 
     3841 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga 
     3901 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 
     3961 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 
     4021 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 
     4081 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 
     4141 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 
     4201 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 
     4261 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct 
     4321 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag 
     4381 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg 
     4441 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc 
     4501 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg 
     4561 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact 
     4621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 
     4681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 
     4741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 
     4801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 
     4861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca 
     4921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 
     4981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 
     5041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 
     5101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 
     5161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 
     5221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 
     5281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 
     5341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 
     5401 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat 
//
