LOCUS       pEF1a-AcGFP1-N1   5483 bp    DNA   circular            17-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631973
FEATURES             Location/Qualifiers
     misc_feature    1348..1422
                     /label=MCS
     CDS             3379..4173
                     /label=Kan(R)/Neo(R)
     rep_origin      4758..5401
                     /label=pUC\origin
     CDS             1430..2146
                     /label=AcGFP1
     rep_origin      complement(2399..2854)
                     /label=f1\origin
     rep_origin      3195..3333
                     /label=SV40\origin
     promoter        12..1346
                     /label=EF1a\Promoter
     polyA_signal    4409..4427
                     /label=HSV\TK\polyA\signals
     polyA_signal    2302..2336
                     /label=SV40\polyA\signal
     misc_feature    3028..3296
                     /label=SV40\early\Promoter
BASE COUNT     1218 a      1487 c      1532 g      1246 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gactcagatc tcgagctcaa 
     1381 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtca tggtgagcaa 
     1441 gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa 
     1501 tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac 
     1561 cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtgaccac 
     1621 cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt 
     1681 cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga 
     1741 cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc gataccctgg tgaatcgcat 
     1801 cgagctgacc ggcaccgatt tcaaggagga tggcaacatc ctgggcaata agatggagta 
     1861 caactacaac gcccacaatg tgtacatcat gaccgacaag gccaagaatg gcatcaaggt 
     1921 gaacttcaag atccgccaca acatcgagga tggcagcgtg cagctggccg accactacca 
     1981 gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac 
     2041 ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct acttcggctt 
     2101 cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtgag cggccgcgac 
     2161 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 
     2221 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 
     2281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     2341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa 
     2401 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 
     2461 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 
     2521 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 
     2581 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 
     2641 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 
     2701 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 
     2761 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 
     2821 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg 
     2881 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 
     2941 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga 
     3001 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 
     3061 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 
     3121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 
     3181 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     3241 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 
     3301 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca 
     3361 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct 
     3421 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 
     3481 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 
     3541 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 
     3601 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 
     3661 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 
     3721 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 
     3781 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 
     3841 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 
     3901 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 
     3961 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 
     4021 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 
     4081 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 
     4141 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 
     4201 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 
     4261 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 
     4321 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa 
     4381 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg 
     4441 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac 
     4501 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa 
     4561 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct 
     4621 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 
     4681 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 
     4741 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 
     4801 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 
     4861 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 
     4921 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 
     4981 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 
     5041 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 
     5101 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 
     5161 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 
     5221 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 
     5281 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 
     5341 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 
     5401 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 
     5461 ataaccgtat taccgccatg cat 
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