LOCUS       pEF1a-AcGFP1-C1   5488 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631974
FEATURES             Location/Qualifiers
     CDS             1370..2086
                     /label=AcGFP1
     rep_origin      complement(2404..2859)
                     /label=f1\origin
     rep_origin      3200..3338
                     /label=SV40\origin
     CDS             3384..4178
                     /label=Kan(R)/Neo(R)
     rep_origin      4763..5406
                     /label=pUC\origin
     polyA_signal    4414..4432
                     /label=HSV\TK\polyA\signals
     polyA_signal    2307..2341
                     /label=SV40\polyA\signal
     promoter        12..1346
                     /label=EF1a\Promoter
     misc_feature    1363..1373
                     /label=Kozak\sequence
     misc_feature    3033..3301
                     /label=SV40\early\Promoter
     misc_feature    2087..2152
                     /label=MCS
     misc_feature    2161..2163
                     /label=Stop
     misc_feature    2165..2167
                     /label=Stop
     misc_feature    2169..2171
                     /label=Stop
BASE COUNT     1221 a      1490 c      1529 g      1248 t 
ORIGIN
        1 tagttattaa tgagtaattc atacaaaagg actcgcccct gccttgggga atcccaggga 
       61 ccgtcgttaa actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc 
      121 cgcccgctct cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca 
      181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 
      241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 
      301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 
      361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 
      421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc 
      481 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 
      541 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg 
      601 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 
      661 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 
      721 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 
      781 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 
      841 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 
      901 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 
      961 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 
     1021 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 
     1081 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 
     1141 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 
     1201 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 
     1261 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 
     1321 tttttcttcc atttcaggtg tcgtgacgct agcgctaccg gtcgccacca tggtgagcaa 
     1381 gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa 
     1441 tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac 
     1501 cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtgaccac 
     1561 cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt 
     1621 cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga 
     1681 cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc gataccctgg tgaatcgcat 
     1741 cgagctgacc ggcaccgatt tcaaggagga tggcaacatc ctgggcaata agatggagta 
     1801 caactacaac gcccacaatg tgtacatcat gaccgacaag gccaagaatg gcatcaaggt 
     1861 gaacttcaag atccgccaca acatcgagga tggcagcgtg cagctggccg accactacca 
     1921 gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac 
     1981 ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct acttcggctt 
     2041 cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtccg gactcagatc 
     2101 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc 
     2161 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 
     2221 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 
     2281 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     2341 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg 
     2401 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
     2461 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
     2521 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
     2581 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
     2641 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
     2701 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
     2761 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
     2821 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 
     2881 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     2941 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 
     3001 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 
     3061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     3121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     3181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     3241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 
     3301 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 
     3361 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     3421 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     3481 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     3541 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 
     3601 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     3661 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     3721 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     3781 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     3841 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     3901 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     3961 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     4021 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     4081 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     4141 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     4201 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     4261 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     4321 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 
     4381 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 
     4441 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 
     4501 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 
     4561 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 
     4621 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     4681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     4741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     4801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     4861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     4921 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     4981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     5041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     5101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     5161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     5221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     5281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     5341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     5401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 
     5461 tgtggataac cgtattaccg ccatgcat 
//
