LOCUS       pDsRed-Monomer-N1   4691 bp    DNA   circular            12-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632465
FEATURES             Location/Qualifiers
     CDS             2587..3381
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter: Enhancer region: 59-465; TATA box: 554-560;Transcription start point: 583; C->G mutation to remove Sac I site: 569"
     rep_origin      complement(1607..2062)
                     /label=f1\origin
                     /note="f1 single-strand DNA origin Packages the noncoding strand of EGFP)"
     rep_origin      2403..2538
                     /label=SV40\origin
                     /note="SV40 origin of replication"
     rep_origin      3966..4609
                     /label=pUC\origin
                     /note="pUC plasmid replication origin"
     CDS             679..1353
                     /label=DsRed-Monomer
     polyA_signal    1510..1515
                     /label=SV40\early\polyA\signal
     polyA_signal    1539..1544
                     /label=SV40\early\polyA\signal
     polyA_signal    3617..3622
                     /label=HSV\TK\polyA\signal
     polyA_signal    3630..3635
                     /label=HSV\TK\polyA\signal
     misc_feature    672..682
                     /label=Kozak\sequence
     misc_feature    2236..2465
                     /label=SV40\Promoter/Enhancer
     misc_feature    2124..2152
                     /label=Kan(R)\Promoter
BASE COUNT     1118 a      1273 c      1245 g      1055 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc 
      721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag 
      781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg 
      841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag 
      901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag 
      961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag 
     1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc 
     1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac 
     1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc 
     1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac 
     1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac 
     1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa 
     1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 
     1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 
     1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 
     1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
     1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
     1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
     1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
     1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
     1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
     1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
     2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 
     2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 
     2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     2521 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg 
     2581 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 
     2641 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 
     2701 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 
     2761 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 
     2821 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 
     2881 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 
     2941 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 
     3001 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 
     3061 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 
     3121 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 
     3181 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 
     3241 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 
     3301 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 
     3361 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 
     3421 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 
     3481 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 
     3541 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 
     3601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc 
     3661 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt 
     3721 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag 
     3781 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat 
     3841 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 
     3901 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 
     3961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 
     4021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 
     4081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 
     4141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 
     4201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 
     4261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 
     4321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
     4381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
     4441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
     4501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
     4561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
     4621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     4681 ccgccatgca t 
//
