LOCUS       pDsRed-Monomer-Hyg-N1   5750 bp    DNA   circular            21-MAR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632494
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter/Enhancer
     misc_feature    591..665
                     /label=MCS
     rep_origin      5025..5668
                     /label=pUC\ori
     rep_origin      complement(1607..2062)
                     /label=f1\ori
     rep_origin      2403..2541
                     /label=SV40\ori
     CDS             4002..4862
                     /label=Amp(R)
     CDS             2560..3585
                     /label=Hyg(R)
     CDS             667..1360
                     /label=DsRed-Monomer
     polyA_signal    1510..1515
                     /label=SV40\early\polyA\signal
     polyA_signal    1539..1544
                     /label=SV40\early\polyA\signal
     polyA_signal    3733..3738
                     /label=SV40\early\polyA\signal
     polyA_signal    3762..3767
                     /label=SV40\early\polyA\signal
     misc_feature    2236..2465
                     /label=SV40\early\Promoter
     misc_feature    672..682
                     /label=Kozak\sequence
BASE COUNT     1440 a      1482 c      1473 g      1355 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcgccaccat ggacaacacc gaggacgtca tcaaggagtt catgcagttc 
      721 aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg cgagggcgag 
      781 ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg cggccccctg 
      841 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc ctacgtgaag 
      901 caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt cacctgggag 
      961 cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc ctccctgcag 
     1021 gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc cgacggcccc 
     1081 gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta cccccaggac 
     1141 ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg ccactacacc 
     1201 tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg caaccactac 
     1261 gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt ggagcagtac 
     1321 gagcacgccg aggcccgcca ctccggctcc cagtagagcg gccgcgactc tagatcataa 
     1381 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 
     1441 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 
     1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     1561 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 
     1621 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 
     1681 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 
     1741 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 
     1801 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 
     1861 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 
     1921 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 
     1981 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 
     2041 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 
     2101 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     2161 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 
     2221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 
     2281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 
     2341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 
     2401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 
     2461 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 
     2521 gaggcttttt tggaggccta ggcttttgca aagatcgata tgaaaaagcc tgaactcacc 
     2581 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag 
     2641 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc 
     2701 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt 
     2761 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg 
     2821 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa 
     2881 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt 
     2941 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg 
     3001 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac 
     3061 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac 
     3121 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac 
     3181 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac 
     3241 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc 
     3301 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc 
     3361 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct 
     3421 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca 
     3481 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat 
     3541 agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagttc tagaggatca 
     3601 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 
     3661 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 
     3721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     3781 tgcctcgcga tccacaacca tggcgaaagg gcctcgtgat acgcctattt ttataggtta 
     3841 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 
     3901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     3961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     4021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     4081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     4141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     4201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     4261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     4321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     4381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     4441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     4501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     4561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     4621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     4681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     4741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     4801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     4861 aaggcgcgcc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 
     4921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     4981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     5041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     5101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     5161 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
     5221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     5281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     5341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     5401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     5461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     5521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     5581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     5641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     5701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat 
//
