LOCUS       pDON-5 Neo Vector 		5828 bp DNA     circular SYN 19-AUG-2021
COMMENT     Created by Takara Bio Inc. http://www.takarabio.com
COMMENT     Cat No. 3657
FEATURES             Location/Qualifiers
     promoter        248..451
                     /label="CMV IE promoter"
     misc_feature    672..1029
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_feature    1370..1418
                     /label="MSC"
                     /note="multiple cloning site"
     intron          1092..1295
                     /label="EF1alpha intron A"
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     exon            1296..1325
                     /label="EF-1-alpha exon"
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     promoter        1472..1682
                     /label="SV40 promoter"
     CDS             1757..2551
                     /label="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
     3'-LTR          2600..3193
                     /label="3'LTR"
     5'-LTR          240..609
                     /label="5'LTR"
     rep_origin      complement(3611..4199)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4370..5230)
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
ORIGIN
        1 aagcttatgt gaaagacccc acctgtaggt ttggcaagct agcgggactt tccattgacg
       61 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaaa tgtatcatat
      121 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      181 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      241 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      301 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      361 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      421 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgcgcca gtcttccgat
      481 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
      541 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
      601 tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac
      661 caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg
      721 actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc
      781 cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccagggac
      841 ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt ttggactctt
      901 tggtgcaccc cccttagagg agggatatgt ggttctggta ggagacgaga acctaaaaca
      961 gttcccgcct ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct
     1021 tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga
     1081 aaatacgcgt gtcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1141 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1201 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1261 agacagtggt tcaaagtttt tttcttccat ttcagccgtc gtgaaaacta cccctaaaag
     1321 ccaaaaaaat cgaattccca aacttaagct tggtaccgag ctcggatctc acgtgggccc
     1381 gcggccgcag atctatcgat ggatccgtcg acgttaacgc atgcatctca attagtcagc
     1441 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1501 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1561 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1621 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1681 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     1741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     1801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     1861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     1921 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     1981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     2281 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     2341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     2401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     2461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     2521 cttctatcgc cttcttgacg agttcttctg actcgaggat aaaataaaag attttattta
     2581 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
     2641 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
     2701 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
     2761 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
     2821 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
     2881 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
     2941 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
     3001 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
     3061 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
     3121 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
     3181 ggggtctttc attgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3241 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3301 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3361 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5341 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     5401 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     5461 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5521 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     5581 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     5641 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     5701 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     5761 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     5821 ccagtgcc
//
