LOCUS       pCMV-RafCAAX   5967 bp    DNA   circular            23-DEC-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631926
FEATURES             Location/Qualifiers
     misc_feature    1..589
                     /label=CMV\IE\Promoter
     misc_feature    608..2682
                     /label=RafCAAX
     misc_feature    2785..2789
                     /label=SV40\early\polyA\signal
     misc_feature    2814..2818
                     /label=SV40\early\polyA\signal
     misc_feature    2882..3337
                     /label=f1\origin
     misc_feature    3399..3427
                     /label=Bacterial\promoter\for\Kan(R)
     misc_feature    3678..3755
                     /label=SV40\origin
     misc_feature    3511..3740
                     /label=SV40\early\Promoter/Enhancer
     misc_feature    3862..4656
                     /label=Kan(R)/Neo(R)
     misc_feature    4892..4897
                     /label=HSV\TK\polyA\signal
     misc_feature    4905..4910
                     /label=HSV\TK\polyA\signal
     misc_feature    5246..5884
                     /label=pUC\origin
BASE COUNT     1524 a      1512 c      1499 g      1432 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagatctc 
      601 gaggccacca tggagcacat acagggagct tggaagacga tcagcaatgg ttttggattc 
      661 aaagatgccg tgtttgatgg ctccagctgc atctctccta caatagttca gcagtttggc 
      721 tatcagcgcc gggcatcaga tgatggcaaa ctcacagatc cttctaagac aagcaacact 
      781 atccgtgttt tcttgccgaa caagcaaaga acagtggtca atgtgcgaaa tggaatgagc 
      841 ttgcatgact gccttatgaa agcactcaag gtgaggggcc tgcaaccaga gtgctgtgca 
      901 gtgttcagac ttctccacga acacaaaggt aaaaaagcac gcttagattg gaatactgat 
      961 gctgcgtctt tgattgaaga agaacttcaa gtagatttcc tggatcatgt tcccctcaca 
     1021 acacacaact ttgctcggaa gacgttcctg aagcttgcct tctgtgacat ctgtcagaaa 
     1081 ttcctgctca atggatttcg atgtcagact tgtggctaca aatttcatga gcactgtagc 
     1141 accaaagtac ctactatgtg tgtggactgg agtaacatca gacaactctt attgtttcca 
     1201 aattccacta ttggtgatag tggagtccca gcactacctt ctttgactat gcgtcgtatg 
     1261 cgagagtctg tttccaggat gcctgttagt tctcagcaca gatattctac acctcacgcc 
     1321 ttcaccttta acacctccag tccctcatct gaaggttccc tctcccagag gcagaggtcg 
     1381 acatccacac ctaatgtcca catggtcagc accaccctgc ctgtggacag caggatgatt 
     1441 gaggatgcaa ttcgaagtca cagcgaatcg gcctcacctt cagccctgtc cagtagcccc 
     1501 aacaatctga gcccaacagg ctggtcacag ccgaaaaccc ccgtgccagc acaaagagag 
     1561 cgggcaccag tatctgggac ccaggagaaa aacaaaatta ggcctcgtgg acagagagat 
     1621 tcaagctatt attgggaaat agaagccagt gaagtgatgc tgtccactcg gattgggtca 
     1681 ggctcttttg gaactgttta taagggtaaa tggcacggag atgttgcagt aaagatccta 
     1741 aaggttgtcg acccaacccc agagcaattc caggccttca ggaatgaggt ggctgttctg 
     1801 cgcaaaacac ggcatgtgaa cattctgctt ttcatggggt acatgacaaa ggacaacctg 
     1861 gcaattgtga cccagtggtg cgagggcagc agcctctaca aacacctgca tgtccaggag 
     1921 accaagtttc agatgttcca gctaattgac attgcccggc agacggctca gggaatggac 
     1981 tatttgcatg caaagaacat catccataga gacatgaaat ccaacaatat atttctccat 
     2041 gaaggcttaa cagtgaaaat tggagatttt ggtttggcaa cagtaaagtc acgctggagt 
     2101 ggttctcagc aggttgaaca acctactggc tctgtcctct ggatggcccc agaggtgatc 
     2161 cgaatgcagg ataacaaccc attcagtttc cagtcggatg tctactccta tggcatcgta 
     2221 ttgtatgaac tgatgacggg ggagcttcct tattctcaca tcaacaaccg agatcagatc 
     2281 atcttcatgg tgggccgagg atatgcctcc ccagatctta gtaagctata taagaactgc 
     2341 cccaaagcaa tgaagaggct ggtagctgac tgtgtgaaga aagtaaagga agagaggcct 
     2401 ctttttcccc agatcctgtc ttccattgag ctgctccaac actctctacc gaagatcaac 
     2461 cggagcgctt ccgagccatc cttgcatcgg gcagcccaca ctgaggatat caatgcttgc 
     2521 acgctgacca cgtccccgag gctgcctgtc ttcgggaatt caaagatgag caaagatggt 
     2581 aaaaagaaga aaaagaagtc aaagacaaac tgtgtaatta tgtaaggatc caccggatct 
     2641 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 
     2701 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 
     2761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 
     2821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc 
     2881 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
     2941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
     3001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
     3061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
     3121 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
     3181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
     3241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
     3301 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa 
     3361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     3421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga 
     3481 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 
     3541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 
     3601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 
     3661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     3721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     3781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga 
     3841 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3901 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     3961 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4021 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac 
     4081 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4141 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     4201 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     4261 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     4321 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     4381 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     4441 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     4501 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     4561 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     4621 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     4681 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     4741 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     4801 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag 
     4861 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt 
     4921 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc 
     4981 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc 
     5041 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata 
     5101 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     5161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     5881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     5941 gtggataacc gtattaccgc catgcat 
//
