LOCUS       pCMV-Raf1   5908 bp    DNA   circular            23-DEC-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631926
FEATURES             Location/Qualifiers
     misc_feature    1..589
                     /label=CMV\IE\Promoter
     misc_feature    619..2565
                     /label=Raf-1
     misc_feature    2726..2730
                     /label=SV40\early\polyA\signal
     misc_feature    2755..2759
                     /label=SV40\early\polyA\signal
     misc_feature    2823..3278
                     /label=f1\origin
     misc_feature    3340..3368
                     /label=Bacterial\promoter\for\Kan(R)
     misc_feature    3619..3696
                     /label=SV40\origin
     misc_feature    3452..3681
                     /label=SV40\early\Promoter/Enhancer
     misc_feature    3803..4597
                     /label=Kan(R)/Neo(R)
     misc_feature    4833..4838
                     /label=HSV\TK\polyA\signal
     misc_feature    4846..4851
                     /label=HSV\TK\polyA\signal
     misc_feature    5187..5825
                     /label=pUC\origin
BASE COUNT     1490 a      1508 c      1485 g      1425 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagatctc 
      601 gagctcaagc ttcgaattca tggagcacat acagggagct tggaagacga tcagcaatgg 
      661 ttttggattc aaagatgccg tgtttgatgg ctccagctgc atctctccta caatagttca 
      721 gcagtttggc tatcagcgcc gggcatcaga tgatggcaaa ctcacagatc cttctaagac 
      781 aagcaacact atccgtgttt tcttgccgaa caagcaaaga acagtggtca atgtgcgaaa 
      841 tggaatgagc ttgcatgact gccttatgaa agcactcaag gtgaggggcc tgcaaccaga 
      901 gtgctgtgca gtgttcagac ttctccacga acacaaaggt aaaaaagcac gcttagattg 
      961 gaatactgat gctgcgtctt tgattggaga agaacttcaa gtagatttcc tggatcatgt 
     1021 tcccctcaca acacacaact ttgctcggaa gacgttcctg aagcttgcct tctgtgacat 
     1081 ctgtcagaaa ttcctgctca atggatttcg atgtcagact tgtggctaca aatttcatga 
     1141 gcactgtagc accaaagtac ctactatgtg tgtggactgg agtaacatca gacaactctt 
     1201 attgtttcca aattccacta ttggtgatag tggagtccca gcactacctt ctttgactat 
     1261 gcgtcgtatg cgagagtctg tttccaggat gcctgttagt tctcagcaca gatattctac 
     1321 acctcacgcc ttcaccttta acacctccag tccctcatct gaaggttccc tctcccagag 
     1381 gcagaggtcg acatccacac ctaatgtcca catggtcagc accaccctgc ctgtggacag 
     1441 caggatgatt gaggatgcaa ttcgaagtca cagcgaatcg gcctcacctt cagccctgtc 
     1501 cagtagcccc aacaatctga gcccaacagg ctggtcacag ccgaaaaccc ccgtgccagc 
     1561 acaaagagag cgggcaccag tatctgggac ccaggagaaa aacaaaatta ggcctcgtgg 
     1621 acagagagat tcaagctatt attgggaaat agaagccagt gaagtgatgc tgtccactcg 
     1681 gattgggtca ggctcttttg gaactgttta taagggtaaa tggcacggag atgttgcagt 
     1741 aaagatccta aaggttgtcg acccaacccc agagcaattc caggccttca ggaatgaggt 
     1801 ggctgttctg cgcaaaacac ggcatgtgaa cattctgctt ttcatggggt acatgacaaa 
     1861 ggacaacctg gcaattgtga cccagtggtg cgagggcagc agcctctaca aacacctgca 
     1921 tgtccaggag accaagtttc agatgttcca gctaattgac attgcccggc agacggctca 
     1981 gggaatggac tatttgcatg caaagaacat catccataga gacatgaaat ccaacaatat 
     2041 atttctccat gaaggcttaa cagtgaaaat tggagatttt ggtttggcaa cagtaaagtc 
     2101 acgctggagt ggttctcagc aggttgaaca acctactggc tctgtcctct ggatggcccc 
     2161 agaggtgatc cgaatgcagg ataacaaccc attcagtttc cagtcggatg tctactccta 
     2221 tggcatcgta ttgtatgaac tgatgacggg ggagcttcct tattctcaca tcaacaaccg 
     2281 agatcagatc atcttcatgg tgggccgagg atatgcctcc ccagatctta gtaagctata 
     2341 taagaactgc cccaaagcaa tgaagaggct ggtagctgac tgtgtgaaga aagtaaagga 
     2401 agagaggcct ctttttcccc agatcctgtc ttccattgag ctgctccaac actctctacc 
     2461 gaagatcaac cggagcgctt ccgagccatc cttgcatcgg gcagcccaca ctgaggatat 
     2521 caatgcttgc acgctgacca cgtccccgag gctgcctgtc ttctagggat ccaccggatc 
     2581 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 
     2641 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 
     2701 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     2761 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg 
     2821 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
     2881 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
     2941 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
     3001 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
     3061 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
     3121 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
     3181 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
     3241 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 
     3301 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     3361 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 
     3421 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 
     3481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
     3541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
     3601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     3661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 
     3721 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 
     3781 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 
     3841 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 
     3901 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 
     3961 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 
     4021 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 
     4081 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 
     4141 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 
     4201 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 
     4261 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 
     4321 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 
     4381 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 
     4441 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 
     4501 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 
     4561 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 
     4621 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 
     4681 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 
     4741 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 
     4801 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 
     4861 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 
     4921 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 
     4981 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 
     5041 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 
     5101 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 
     5161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     5221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     5281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     5341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     5401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     5461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     5521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     5581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     5641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     5701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     5761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     5821 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 
     5881 tgtggataac cgtattaccg ccatgcat 
//
