LOCUS       pCMV-E2-Crimson   4638 bp    DNA   circular            28-JAN-2010
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632556
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
                     /note="Human cytomegalovirus (CMV) immediate early promoter"
     CDS             2534..3328
                     /label=Kan(R)/Neo(R)
     rep_origin      3913..4556
                     /label=pUC\ori
     polyA_signal    3564..3582
                     /label=HSV\TK\poly\A
     CDS             628..1305
                     /label=E2-Crimson
     misc_signal     621..631
                     /label=Kozak\sequence
     misc_signal     1303..1305
                     /label=Stop\codon
     misc_feature    1457..1462
                     /label=SV40\early\polyA\signal
     misc_feature    complement(1554..2009)
                     /label=f1\ori
     misc_feature    2350..2488
                     /label=SV40\ori
     misc_feature    1486..1491
                     /label=SV40\early\polyA\signal
BASE COUNT     1103 a      1258 c      1221 g      1056 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atccaccggt cgccaccatg gatagcactg agaacgtcat caagcccttc 
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc 
      721 gtgggcgagg gcaagcccta cgagggcacc cagaccgcca agctgcaagt gaccaagggc 
      781 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcttctacgg ctccaaggcg 
      841 tacatcaagc accccgccga catccccgac tacctcaagc agtccttccc cgagggcttc 
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc 
      961 tccctgcagg acggcaccct catctaccac gtgaagttca tcggcgtgaa cttcccctcc 
     1021 gacggccccg taatgcagaa gaagactctg ggctgggagc cctccactga gcgcaactac 
     1081 ccccgcgacg gcgtgctgaa gggcgagaac cacatggcgc tgaagctgaa gggcggcggc 
     1141 cactacctgt gtgagttcaa gtccatctac atggccaaga agcccgtgaa gctgcccggc 
     1201 taccactacg tggactacaa gctcgacatc acctcccaca acgaggacta caccgtggtg 
     1261 gagcagtacg agcgcgccga ggcccgccac cacctgttcc agtagcggcc gcgactctag 
     1321 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 
     1381 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 
     1441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     1501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt 
     1561 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 
     1621 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 
     1681 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 
     1741 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 
     1801 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 
     1861 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 
     1921 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 
     1981 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 
     2041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
     2101 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 
     2161 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
     2221 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     2281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     2341 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     2401 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     2461 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg 
     2521 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 
     2581 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 
     2641 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 
     2701 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 
     2761 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 
     2821 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 
     2881 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 
     2941 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 
     3001 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 
     3061 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 
     3121 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 
     3181 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 
     3241 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 
     3301 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 
     3361 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 
     3421 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 
     3481 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg 
     3541 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg 
     3601 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 
     3661 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 
     3721 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt 
     3781 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     3841 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     3901 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     3961 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     4021 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     4081 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     4141 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     4201 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     4261 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     4321 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     4381 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     4441 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     4501 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     4561 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     4621 cgtattaccg ccatgcat 
//
