LOCUS       pBE-S DNA Vector                5938 bp DNA     circular SYN 29-OCT-2021
COMMENT     Created by Takara Bio http://www.takarabio.com
COMMENT     Cat. No. 3380
FEATURES             Location/Qualifiers
     misc_feature    198..284
                     /label="aprE SP"
                     /note="B. subtilis-derived secretory signal peptide"
     misc_feature    285..356
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             357..374
                     /label="6xHis"
                     /note="6xHis affinity tag"
     rep_origin      complement(1700..2288)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2459..3319)
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(3320..3424)
                     /label="AmpR promoter"
     CDS             complement(3594..4355)
                     /label="KanR"
                     /note="Kanamycin resistance gene, selection marker in B. 
                     subtilis"
     rep_origin      complement(4524..5528)
		     /direction=LEFT
                     /label="pUB ori"
                     /note="pUB110-derived replication ori"

ORIGIN
        1 actagtgttc ttttctgtat gaaaatagtt atttcgagtc tctacggaaa tagcgagaga
       61 tgatatacct aaatagagat aaaatcatct caaaaaaatg ggtctactaa aatattattc
      121 catctattac aataaattca cagaatagtc ttttaagtaa gtctactctg aacttaagca
      181 aaaggagagg gacgcgtgtg agaagcaaaa aattgtggat cagcttgttg tttgcgttaa
      241 cgttaatctt tacgatggcg ttcagcaaca tgtctgcgca ggctgcggcc ggtgcacata
      301 tggagctcgg taccctcgag ggatccgaat tcaagcttgt cgacctgcag tctagacatc
      361 accatcatca ccactaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
      421 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
      481 taggcatagg cttggttatg ccggtactgc cgggcctatt tcactttttg cattctacaa
      541 actgcataac tattatgtaa atcgctcctt tttaggtggc acaaatgtga ggcattttcg
      601 ctctttccgg caaccacttc caagtaaagt ataacacact atactttata ttcataaagt
      661 gtgtgctctg cgaggctgtc ggcagtgccg accaaaacca taaaaccttt aagacctttc
      721 ttttttttac gagaaaaaag aaacaaaaaa acctgccctc tgccacctca gcaaaggggg
      781 gttttgctct cgtgctcgtt taaaaatcag caagggacag gtagtatttt ttgagaagat
      841 cactcaaaaa atctccacct ttaaaccctt gccaattttt attttgtccg ttttgtctag
      901 cttaccgaaa gccagactca gcaagaataa aatttttatt gtctttcggt tttctagtgt
      961 aacggacaaa accactcaaa ataaaaaaga tacaagagag gtctctcgta tcttttattc
     1021 agcaatcgcg cccgattgct gaacagatta ataatagatt ttagcttttt atttgttgaa
     1081 aaaagctaat caaattgttg tcgggatcaa ttactgcaaa gtctcgttca tcccaccact
     1141 gatcttttaa tgatgtattg gggtgcaaaa tgcccaaagg cttaatatgt tgatataatt
     1201 catcaattcc ctctacttca atgcggcaac tagcagtacc agcaataaac gactccgcac
     1261 ctgtacaaac cggtgaatca ttactacgag agcgccagcc ttcatcactt gcctcccata
     1321 gatgaatccg aacctcatta cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag
     1381 tgaaacctag tttatcgcaa taaaaaccta tactcttttt aatatccccg actggcaatg
     1441 ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta atgtaaatct
     1501 attaccttat tattaattca attcgctcat aattaatcct ttttcttatt acgcaaaatg
     1561 gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg ataattacta
     1621 atactaggag aagttaataa atacgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1681 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1741 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1801 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1861 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1921 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1981 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2041 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2101 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2161 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2221 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2281 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2341 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2401 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2461 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2521 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2581 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2641 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2701 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2761 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2821 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2881 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2941 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3001 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3061 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3121 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3181 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3241 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3301 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3361 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3421 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     3481 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     3541 gtgaaaacct ctgacagtaa ccaacatgat taacaattat tagaggtcat cgttcaaaat
     3601 ggtatgcgtt ttgacacatc cactatatat ccgtgtcgtt ctgtccactc ctgaatccca
     3661 ttccagaaat tctctagcga ttccagaagt ttctcagagt cggaaagttg accagacatt
     3721 acgaactggc acagatggtc ataacctgaa ggaagatctg attgcttaac tgcttcagtt
     3781 aagaccgaag cgctcgtcgt ataacagatg cgatgatgca gaccaatcaa catggcacct
     3841 gccattgcta cctgtacagt caaggatggt agaaatgttg tcggtccttg cacacgaata
     3901 ttacgccatt tgcctgcata ttcaaacagc tcttctacga taagggcaca aatcgcatcg
     3961 tggaacgttt gggcttctac cgatttagca gtttgataca ctttctctaa gtatccacct
     4021 gaatcataaa tcggcaaaat agagaaaaat tgaccatgtg taagcggcca atctgattcc
     4081 acctgagatg cataatctag tagaatctct tcgctatcaa aattcacttc caccttccac
     4141 tcaccggttg tccattcatg gctgaactct gcttcctctg ttgacatgac acacatcatc
     4201 tcaatatccg aatagggccc atcagtctga cgaccaagag agccataaac accaatagcc
     4261 ttaacatcat ccccatattt atccaatatt cgttccttaa tttcatgaac aatcttcatt
     4321 ctttcttctc tagtcattat tattggtcca ttcactattc tcattccctt ttcagataat
     4381 tttagatttg cttttctaaa taagaatatt tggagagcac cgttcttatt cagctattaa
     4441 taactcgtct tcctaagcat ccttcaatcc ttttaataac aattatagca tctaatcttc
     4501 aacaaactgg cccgtttgtt gaactactct ttaataaaat aatttttccg ttcccaattc
     4561 cacattgcaa taatagaaaa tccatcttca tcggcttttt cgtcatcatc tgtatgaatc
     4621 aaatcgcctt cttctgtgtc atcaaggttt aattttttat gtatttcttt taacaaacca
     4681 ccataggaga ttaacctttt acggtgtaaa ccttcctcca aatcagacaa acgtttcaaa
     4741 ttcttttctt catcatcggt cataaaatcc gtatccttta caggatattt tgcagtttcg
     4801 tcaattgccg attgtatatc cgatttatat ttatttttcg gtcgaatcat ttgaactttt
     4861 acatttggat catagtctaa tttcattgcc tttttccaaa attgaatcca ttgtttttga
     4921 ttcacgtagt tttctgtatt cttaaaataa gttggttcca cacataccaa tacatgcatg
     4981 tgctgattat aagaattatc tttattattt attgtcactt ccgttgcacg cataaaacca
     5041 acaagatttt tattaatttt tttatattgc atcattcggc gaaatccttg agccatatct
     5101 gacaaactct tatttaattc ttcgccatca taaacatttt taactgttaa tgtgagaaac
     5161 aaccaacgaa ctgttggctt ttgtttaata acttcagcaa caaccttttg tgactgaatg
     5221 ccatgtttca ttgctctcct ccagttgcac attggacaaa gcctggattt acaaaaccac
     5281 actcgataca actttctttc gcctgtttca cgattttgtt tatactctaa tatttcagca
     5341 caatctttta ctctttcagc ctttttaaat tcaagaatat gcagaagttc aaagtaatca
     5401 acattagcga ttttcttttc tctccatggt ctcacttttc cactttttgt cttgtccact
     5461 aaaacccttg atttttcatc tgaataaatg ctactattag gacacataat attaaaagaa
     5521 acccccatct atttagttat ttgtttagtc acttataact ttaacagatg gggtttttct
     5581 gtgcaaccaa ttttaagggt tttcaatact ttaaaacaca tacataccaa cacttcaacg
     5641 cacctttcag caactaaaat aaaaatgacg ttatttctat atgtatcaag ataagaaaga
     5701 acaagttcaa aaccatcaaa aaaagacacc ttttcaggtg ctttttttat tttataaact
     5761 cattccctga tctcgacttc gttctttttt tacctctcgg ttatgagtta gttcaaattc
     5821 gttcttttta ggttctaaat cgtgtttttc ttggaattgt gctgttttat cctttacctt
     5881 gtctacaaac cccttaaaaa cgtttttaaa ggcttttaag ccgtctgtac gttcctaa
//
