LOCUS       pBApo-CMV Vector 		3447 bp DNA     circular SYN 19-AUG-2021
COMMENT     Created by Takara Bio Inc. http://www.takarabio.com
COMMENT     Cat No. 3242
FEATURES             Location/Qualifiers
     promoter        60..567
                     /label="CMV IE promoter"
     polyA_signal    674..721
                     /label="HSV TK poly(A) signal"
     primer_bind     complement(808..824)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1403..2263
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3408..3424
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    580..620
                     /label="MCS"
                     /note="multiple cloning site"
ORIGIN
        1 gaattctaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
       61 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      121 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      181 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      241 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      301 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      361 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      421 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      481 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      541 cggtgggagg tctatataag cagagctggt ttagtgaacc ggatcctcta gagtcgacct
      601 gcaggcatgc aagcttgggg gaggctaact gaaacacgga aggagacaat accggaagga
      661 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
      721 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
      781 tggggagctt gatatcgata gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
      841 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      901 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      961 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     1021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2101 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3181 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3241 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3301 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3361 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3421 tgaccatgat tacgaattat cgatatc
//
