LOCUS       pAmCyan1-C1   4701 bp    DNA   circular            20-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632441,630050
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     rep_origin      2413..2548
                     /label=SV40\origin
     CDS             2597..3389
                     /label=Kan(R)/Neo(R)
                     /note="Kanamycin/neomycin resistance gene Neomycin phosphotransferase coding sequences: start codon (ATG): 2629-2631; stop codon: 3421-3423; G to A mutation to remove Pst I site: 2811; C to A mutation to remove BssH II site: 3157"
     rep_origin      3976..4619
                     /label=pUC\origin
     promoter        2246..2514
                     /label=SV40\early\Promoter
     polyA_signal    1520..1570
                     /label=SV40\early\polyA\signal
     polyA_signal    3627..3645
                     /label=HSV\TK\polyA\signal
     promoter        2134..2169
                     /label=Kan(R)\Promoter
     rep_origin      complement(1617..2072)
                     /label=f1\origin
     CDS             613..1299
                     /label=AmCyan1
BASE COUNT     1116 a      1293 c      1225 g      1067 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggccct gtccaacaag ttcatcggcg acgacatgaa gatgacctac 
      661 cacatggacg gctgcgtgaa cggccactac ttcaccgtga agggcgaggg cagcggcaag 
      721 ccctacgagg gcacccagac ctccaccttc aaggtgacca tggccaacgg cggccccctg 
      781 gccttctcct tcgacatcct gtccaccgtg ttcatgtacg gcaaccgctg cttcaccgcc 
      841 taccccacca gcatgcccga ctacttcaag caggccttcc ccgacggcat gtcctacgag 
      901 agaaccttca cctacgagga cggcggcgtg gccaccgcca gctgggagat cagcctgaag 
      961 ggcaactgct tcgagcacaa gtccaccttc cacggcgtga acttccccgc cgacggcccc 
     1021 gtgatggcca agaagaccac cggctgggac ccctccttcg agaagatgac cgtgtgcgac 
     1081 ggcatcttga agggcgacgt gaccgccttc ctgatgctgc agggcggcgg caactacaga 
     1141 tgccagttcc acacctccta caagaccaag aagcccgtga ccatgccccc caaccacgtg 
     1201 gtggagcacc gcatcgccag aaccgacctg gacaagggcg gcaacagcgt gcagctgacc 
     1261 gagcacgccg tggcccacat cacctccgtg gtgcccttct ccggactcag atctcgagct 
     1321 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatccaccgg atctagataa 
     1381 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca 
     1441 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt 
     1501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     1561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat 
     1621 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 
     1681 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 
     1741 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 
     1801 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 
     1861 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 
     1921 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 
     1981 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 
     2041 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg 
     2101 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     2161 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac 
     2221 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga 
     2281 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc 
     2341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc 
     2401 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
     2461 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
     2521 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg 
     2581 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 
     2641 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 
     2701 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 
     2761 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt 
     2821 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 
     2881 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 
     2941 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 
     3001 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 
     3061 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 
     3121 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 
     3181 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 
     3241 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 
     3301 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 
     3361 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 
     3421 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 
     3481 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 
     3541 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata 
     3601 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg 
     3661 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg 
     3721 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt 
     3781 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca 
     3841 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 
     3901 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 
     3961 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 
     4021 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 
     4081 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 
     4141 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 
     4201 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 
     4261 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 
     4321 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 
     4381 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 
     4441 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 
     4501 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 
     4561 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 
     4621 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 
     4681 aaccgtatta ccgccatgca t 
//
