LOCUS       pAcGFP1-Tubulin   6045 bp    DNA   circular            21-MAR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632488
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     CDS             613..1329
                     /label=AcGFP1
     rep_origin      complement(2961..3416)
                     /label=f1\origin
     rep_origin      3757..3895
                     /label=SV40\origin
     CDS             3941..4735
                     /label=Kan(R)/Neo(R)
     rep_origin      5320..5963
                     /label=pUC\origin
     polyA_signal    2864..2869
                     /label=SV40\early\polyA\signal
     polyA_signal    2893..2898
                     /label=SV40\early\polyA\signal
     polyA_signal    4971..4976
                     /label=HSV\TK\polyA\signal
     polyA_signal    4984..4989
                     /label=HSV\TK\polyA\signal
     CDS             1351..2700
                     /label=Tubulin
     misc_feature    606..616
                     /label=Kozak\sequence
     misc_feature    3590..3819
                     /label=SV40\early\Promoter
     promoter        3478..3506
                     /label=Kan(R)\Promoter
BASE COUNT     1441 a      1631 c      1581 g      1392 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 
      661 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 
      781 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 
      841 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 
      901 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 
      961 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 
     1021 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 
     1081 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 
     1141 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 
     1201 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 
     1261 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 
     1321 ctgtacaagt ccggactcag atctcgagtg cgtgagtgca tctccatcca cgttggccag 
     1381 gctggtgtcc agattggcaa tgcctgctgg gagctctact gcctggaaca cggcatccag 
     1441 cccgatggcc agatgccaag tgacaagacc attgggggag gagatgactc cttcaacacc 
     1501 ttcttcagtg agacgggcgc tggcaagcac gtgccccggg ctgtgtttgt agacttggaa 
     1561 cccacagtca ttgatgaagt tcgcactggc acctaccgcc agctcttcca ccctgagcag 
     1621 ctcatcacag gcaaggaaga tgctgccaat aactatgccc gagggcacta caccattggc 
     1681 aaggagatca ttgaccttgt gttggaccga attcgcaagc tggctgacca gtgcaccggt 
     1741 cttcagggct tcttggtttt ccacagcttt ggtgggggaa ctggttctgg gttcacctcc 
     1801 ctgctcatgg aacgtctctc agttgattat ggcaagaagt ccaagctgga gttctccatt 
     1861 tacccagcac cccaggtttc cacagctgta gttgagccct acaactccat cctcaccacc 
     1921 cacaccaccc tggagcactc tgattgtgcc ttcatggtag acaatgaggc catctatgac 
     1981 atctgtcgta gaaacctcga tatcgagcgc ccaacctaca ctaaccttaa ccgccttatt 
     2041 agccagattg tgtcctccat cactgcttcc ctgagatttg atggagccct gaatgttgac 
     2101 ctgacagaat tccagaccaa cctggtgccc tacccccgca tccacttccc tctggccaca 
     2161 tatgcccctg tcatctctgc tgagaaagcc taccatgaac agctttctgt agcagagatc 
     2221 accaatgctt gctttgagcc agccaaccag atggtgaaat gtgaccctcg ccatggtaaa 
     2281 tacatggctt gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt caatgctgcc 
     2341 attgccacca tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc cactggcttc 
     2401 aaggttggca tcaactacca gcctcccact gtggtgcctg gtggagacct ggccaaggta 
     2461 cagagagctg tgtgcatgct gagcaacacc acagccattg ctgaggcctg ggctcgcctg 
     2521 gaccacaagt ttgacctgat gtatgccaag cgtgcctttg ttcactggta cgtgggtgag 
     2581 gggatggagg aaggcgagtt ttcagaggcc cgtgaagata tggctgccct tgagaaggat 
     2641 tatgaggagg ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg agaggaatac 
     2701 taaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt 
     2761 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca 
     2821 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     2881 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     2941 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 
     3001 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 
     3061 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 
     3121 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 
     3181 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 
     3241 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 
     3301 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 
     3361 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg 
     3421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     3481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 
     3541 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 
     3601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     3661 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     3721 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     3781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     3841 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     3901 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     3961 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     4021 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     4081 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct 
     4141 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     4201 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     4261 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     4321 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     4381 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     4441 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac 
     4501 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     4561 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     4621 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     4681 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     4741 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     4801 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     4861 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta 
     4921 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga 
     4981 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 
     5041 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 
     5101 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 
     5161 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 
     5221 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     5281 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     5341 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     5401 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     5461 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     5521 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     5581 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     5641 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     5701 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     5761 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     5821 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     5881 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     5941 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     6001 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 
//
