LOCUS       pAcGFP1-N_In-Fusion_Ready    4688 bp    DNA   circular     11-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632501
FEATURES             Location/Qualifiers
     promoter        4078..4666
                     /label=CMV\IE\Promoter
     rep_origin      3352..3995
                     /label=pUC\origin
     CDS             1973..2767
                     /label=Kan(R)/Neo(R)
     enhancer        1622..1832
                     /label=SV40\early\promoter\enhancer
     rep_origin      1789..1924
                     /label=SV40\ori
     polyA_signal    3003..3021
                     /label=HSV\TK\polyA
     polyA_signal    896..930
                     /label=SV40\polyA\signal
     CDS             30..740
                     /label=AcGFP1
     misc_feature    4688..1
                     /label=In-Fusion\Cloning\Site
BASE COUNT     1130 a      1254 c      1228 g      1076 t 
ORIGIN
        1 agcttgcgaa ttctggccta ccggtcgcga gcaagggcgc cgagctgttc accggcatcg 
       61 tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc gtgagcggcg 
      121 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
      181 agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg cagtgcttct 
      241 cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg cctgagggct 
      301 acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg cgcgccgagg 
      361 tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc gatttcaagg 
      421 aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac aatgtgtaca 
      481 tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc cacaacatcg 
      541 aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc ggcgatggcc 
      601 ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc aaggacccca 
      661 acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc atcacccacg 
      721 gcatggatga gctgtacaag tgagcggccg cgactctaga tcataatcag ccataccaca 
      781 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 
      841 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 
      901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
      961 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa 
     1021 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 
     1081 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 
     1141 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 
     1201 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 
     1261 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 
     1321 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 
     1381 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 
     1441 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     1501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     1561 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 
     1621 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     1681 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 
     1741 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 
     1801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     1861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     1921 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 
     1981 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     2041 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     2101 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 
     2161 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     2221 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     2281 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     2341 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     2401 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     2461 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 
     2521 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     2581 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     2641 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     2701 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     2761 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 
     2821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     2881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 
     2941 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 
     3001 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 
     3061 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 
     3121 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 
     3181 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 
     3241 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 
     3301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 
     3361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 
     3421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 
     3481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 
     3541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 
     3601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 
     3661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 
     3721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 
     3781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 
     3841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 
     3901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 
     3961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 
     4021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag 
     4081 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 
     4141 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 
     4201 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 
     4261 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 
     4321 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 
     4381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 
     4441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 
     4501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 
     4561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg 
     4621 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agctaaggcc 
     4681 tctgtcga 
//
