LOCUS       pAcGFP1-N2   4730 bp    DNA   circular             9-DEC-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632485,632483
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     misc_feature    591..665
                     /label=MCS
     CDS             2626..3417
                     /label=Kan(R)/Neo(R)
     misc_feature    4005..4648
                     /label=pUC\origin
     CDS             677..1393
                     /label=AcGFP1
     misc_feature    1550..1584
                     /label=SV40\early\polyA\signal
     misc_feature    1647..2102
                     /label=f1\origin
     misc_feature    2442..2577
                     /label=SV40\origin
     misc_feature    3656..3674
                     /label=HSV\TK\polyA\signal
     promoter        2163..2191
                     /label=Bacterial\Promoter\for\Kan(R)
     misc_feature    2275..2504
                     /label=SV40\early\Promoter/Enhancer
BASE COUNT     1138 a      1269 c      1242 g      1081 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg ccggtcatgg tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat 
      721 cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga 
      781 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 
      841 tgtgccctgg cccaccctgg tgaccaccct gagctacggc gtgcagtgct tctcacgcta 
      901 ccccgatcac atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacatcca 
      961 ggagcgcacc atcttcttcg aggatgacgg caactacaag tcgcgcgccg aggtgaagtt 
     1021 cgagggcgat accctggtga atcgcatcga gctgaccggc accgatttca aggaggatgg 
     1081 caacatcctg ggcaataaga tggagtacaa ctacaacgcc cacaatgtgt acatcatgac 
     1141 cgacaaggcc aagaatggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg 
     1201 cagcgtgcag ctggccgacc actaccagca gaataccccc atcggcgatg gccctgtgct 
     1261 gctgcccgat aaccactacc tgtccaccca gagcgccctg tccaaggacc ccaacgagaa 
     1321 gcgcgatcac atgatctact tcggcttcgt gaccgccgcc gccatcaccc acggcatgga 
     1381 tgagctgtac aagtgagcgg ccgcgactct agatcataat cagccatacc acatttgtag 
     1441 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 
     1501 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 
     1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 
     1621 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg 
     1681 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 
     1741 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 
     1801 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 
     1861 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 
     1921 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 
     1981 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 
     2041 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 
     2101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 
     2161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 
     2221 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg 
     2281 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
     2341 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 
     2401 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 
     2461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     2521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     2581 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 
     2641 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 
     2701 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 
     2761 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg 
     2821 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 
     2881 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 
     2941 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 
     3001 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 
     3061 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 
     3121 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc 
     3181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 
     3241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 
     3301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 
     3361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 
     3421 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 
     3481 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 
     3541 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga 
     3601 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa 
     3661 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 
     3721 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 
     3781 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 
     3841 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa 
     3901 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
     3961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
     4021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
     4081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
     4141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 
     4201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
     4261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
     4321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
     4381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
     4441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
     4501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
     4561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
     4621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 
     4681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat 
//
