LOCUS       pAcGFP1-Hyg-N1   5785 bp    DNA   circular            11-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632489
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     misc_feature    594..672
                     /label=MCS
     rep_origin      5060..5703
                     /label=pUC\origin
     CDS             673..1389
                     /label=AcGFP1
     rep_origin      complement(1642..2097)
                     /label=f1\origin
     CDS             4037..4897
                     /label=Amp(R)
     CDS             2595..3620
                     /label=Hyg(R)
     rep_origin      2438..2576
                     /label=SV40\origin
     polyA_signal    1545..1550
                     /label=SV40\early\polyA\signal
     polyA_signal    1574..1579
                     /label=SV40\early\polyA\signal
     polyA_signal    3768..3773
                     /label=SV40\early\polyA\signal
     polyA_signal    3797..3802
                     /label=SV40\early\polyA\signal
     misc_feature    2271..2500
                     /label=SV40\early\Promoter
BASE COUNT     1459 a      1475 c      1469 g      1382 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 
      661 gatccaccgg tcatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 
      721 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 
      781 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 
      841 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 
      901 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 
      961 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 
     1021 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 
     1081 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 
     1141 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 
     1201 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 
     1261 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 
     1321 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 
     1381 ctgtacaagt gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt 
     1441 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 
     1501 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 
     1561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 
     1621 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 
     1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata 
     1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac 
     1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc 
     1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 
     1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 
     1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg 
     2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag 
     2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa 
     2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     2581 ttgcaaagat cgatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga 
     2641 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg 
     2701 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg 
     2761 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg 
     2821 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac 
     2881 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg 
     2941 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat 
     3001 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg 
     3061 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc 
     3121 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc 
     3181 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg 
     3241 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga 
     3301 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc 
     3361 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc 
     3421 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa 
     3481 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg 
     3541 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc 
     3601 gtccgagggc aaaggaatag agttctagag gatcataatc agccatacca catttgtaga 
     3661 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 
     3721 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 
     3781 catcacaaat ttcacaaata aagcattttt ttcactgcct cgcgatccac aaccatggcg 
     3841 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 
     3901 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     3961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     4021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     4081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     4141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     4201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     4261 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     4321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     4381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     4441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     4501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     4561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     4621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     4681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     4741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     4801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     4861 cgctgagata ggtgcctcac tgattaagca ttggtaaggc gcgccaggcc ctgccatagc 
     4921 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
     4981 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
     5041 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
     5101 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
     5161 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
     5221 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
     5281 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
     5341 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
     5401 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
     5461 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
     5521 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
     5581 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
     5641 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
     5701 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
     5761 ggataaccgt attaccgcca tgcat 
//
