LOCUS       pAcGFP1-F_Hyg   5865 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632510
FEATURES             Location/Qualifiers
     misc_feature    1345..1407
                     /label=c-Ha-Ras\farnesylation\signal
     CDS             613..1329
                     /label=AcGFP1
     misc_feature    606..616
                     /label=Kozak\sequence
     promoter        1..589
                     /label=CMV\IE\Promoter
     rep_origin      complement(1722..2177)
                     /label=f1\origin
     rep_origin      2518..2656
                     /label=SV40\origin
     rep_origin      5140..5783
                     /label=pUC\origin
     CDS             2675..3700
                     /label=Hyg(R)
     CDS             4117..4977
                     /label=Amp(R)
     polyA_signal    1625..1630
                     /label=SV40\early\polyA\signal
     polyA_signal    1654..1659
                     /label=SV40\early\polyA\signal
     polyA_signal    3848..3853
                     /label=SV40\early\polyA\signal
     polyA_signal    3877..3882
                     /label=SV40\early\polyA\signal
     misc_feature    2574..2580
                     /label=SV40\early\promoter
     promoter        4047..4075
                     /label=Amp(R)\Promoter
BASE COUNT     1477 a      1499 c      1488 g      1401 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 
      661 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 
      781 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 
      841 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 
      901 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 
      961 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 
     1021 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 
     1081 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 
     1141 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 
     1201 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 
     1261 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 
     1321 ctgtacaagt ccggactcag atctaagctg aaccctcctg atgagagtgg ccccggctgc 
     1381 atgagctgca agtgtgtgct ctcctgagga tccagatctc gagctcaagc ttcgaattct 
     1441 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc 
     1501 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 
     1561 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 
     1621 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     1681 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 
     1741 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 
     1801 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 
     1861 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 
     1921 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 
     1981 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 
     2041 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 
     2101 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 
     2161 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     2221 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     2281 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 
     2341 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     2401 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 
     2461 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 
     2521 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     2581 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     2641 ttttttggag gcctaggctt ttgcaaagat cgatatgaaa aagcctgaac tcaccgcgac 
     2701 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 
     2761 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 
     2821 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 
     2881 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 
     2941 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 
     3001 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca 
     3061 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 
     3121 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 
     3181 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 
     3241 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 
     3301 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 
     3361 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 
     3421 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 
     3481 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 
     3541 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 
     3601 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 
     3661 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agttctagag gatcataatc 
     3721 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 
     3781 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 
     3841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcct 
     3901 cgcgatccac aaccatggcg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 
     3961 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 
     4021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     4081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 
     4141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 
     4201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 
     4261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 
     4321 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa 
     4381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 
     4441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 
     4501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 
     4561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 
     4621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 
     4681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 
     4741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 
     4801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 
     4861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 
     4921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaaggc 
     4981 gcgccaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt 
     5041 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 
     5101 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 
     5161 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 
     5221 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 
     5281 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 
     5341 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 
     5401 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 
     5461 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 
     5521 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 
     5581 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 
     5641 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 
     5701 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 
     5761 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 
     5821 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat 
//
