LOCUS       pAcGFP1-C1   4731 bp    DNA   circular            13-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 632470,632426
FEATURES             Location/Qualifiers
     promoter        1..589
                     /label=CMV\IE\Promoter
     CDS             613..1329
                     /label=AcGFP1
     rep_origin      1647..2102
                     /label=f1\ori
     rep_origin      2443..2581
                     /label=SV40\origin
     CDS             2627..3421
                     /label=Kan(R)/Neo(R)
     rep_origin      4006..4649
                     /label=pUC\origin
     polyA_signal    1550..1555
                     /label=SV40\early\polyA\signal
     polyA_signal    1579..1584
                     /label=SV40\early\polyA\signal
     polyA_signal    3657..3662
                     /label=HSV\TK\polyA\signal
     polyA_signal    3670..3675
                     /label=HSV\TK\polyA\signal
     misc_feature    606..616
                     /label=Kozak\sequence
     misc_feature    1330..1407
                     /label=MCS
     misc_feature    2276..2505
                     /label=SV40\early\Promoter/Enhancer
     promoter        2164..2192
                     /label=Kan(R)\Promoter
BASE COUNT     1141 a      1270 c      1237 g      1083 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg 
      661 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg 
      781 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc 
      841 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag 
      901 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag 
      961 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac 
     1021 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac 
     1081 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc 
     1141 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg 
     1201 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc 
     1261 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag 
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc 
     1381 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta 
     1441 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 
     1501 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     1561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     1621 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc 
     1681 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 
     1741 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 
     1801 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 
     1861 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 
     1921 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 
     1981 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 
     2041 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 
     2101 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     2161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     2221 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 
     2281 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     2341 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     2401 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 
     2461 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 
     2521 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 
     2581 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     2641 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     2701 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     2761 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 
     2821 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     2881 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     2941 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     3001 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     3061 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     3121 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 
     3181 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     3241 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     3301 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     3361 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     3421 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 
     3481 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 
     3541 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 
     3601 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 
     3661 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 
     3721 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 
     3781 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 
     3841 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 
     3901 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     3961 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     4021 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     4081 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     4141 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     4201 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     4261 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     4321 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     4381 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     4441 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     4501 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     4561 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     4621 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     4681 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t 
//
